附子对虚寒证、知母对虚热证大鼠肝全基因表达谱影响.pdf

28 条，其中 16 条为免疫应答相关基因。 结论：虚寒证与虚热证模型动物的主要症状及体征与虚寒证与虚热证的临床诊 断基本相符，体现了虚寒证“阳虚内寒”和虚热证“阴虚内热”的病理特点。虚寒 证可能通过多种刺激应答相关基因的下调，导致免疫应答功能、防御应答功能及对 其他生物体刺激应答功能的降低，虚寒证“阳气不足”的分子机制可能与此类基因 的异常表达相关；附子对虚寒证大鼠免疫应答相关基因及氧化还原酶活性相关基因 的调控，可能是附子“温阳散寒”作用的分子机制。虚热证“阴虚内热”的分子机 制可能与刺激应答相关基因及固醇代谢相关基因两类基因的异常表达相关；知母对 虚热证大鼠刺激应答相关基因表达的调控，可能是知母“滋阴”作用的分子机制。 虚寒证与虚热证大鼠均出现了免疫应答相关基因表达的显著改变，这可能是虚寒证 与虚热证大鼠“精气夺则虚”的分子机制。虚寒证与虚热证虽然同为虚证，但有“寒 证”和“热证”不同的表现，氧化还原酶活性相关基因的异常表达可能是虚寒证与 虚热证出现不同症状的分子机制。 The Influence of Aconitum carmichaeli on Asthenia Cold Syndrome Rats and Anemarrhenae Rhizoma on Asthenia Hot Syndrome Rats on Whole Genome Expression of Liver Specially: Theoretical and experimental research on Integrated Traditional and Western Medicine Author:Han Bingbing Tutor: Wang Shijun Objective: To discuss asthenia cold syndrome rat models and asthenia hot syndrome rat models and the evaluation system to superficial characteristics fit in with throry of Traditional Chinese Medicine, the Asthenia cold syndrome rat models and asthenia hot syndrome rat models were induced by compound preparation of traditional chinese medicine, which were estimated by PLS data analysis. To analyse the molecular mechanism and the related mechanism of property and effect of Chinese medicinal herbs with hot and cold property from the view of gene expression spectrum,we study the influence of Chinese medicinal herbs with hot property such as Aconitum carmichaeli on asthenia cold syndrome rats, herbs with cold property such as Anemarrhenae Rhizoma on asthenia hot syndrome rats on whole genome expression of liver by gene chip. Methods: The rat models were induced by compound preparation of traditional chinese medicine. Asthenia cold syndrome rats were induced by compound preparation of Raw Gypsum, Radix gentianae, Cortex Phellodendri,