中山大学孙逸仙纪念医院儿科.pptVIP

* * The Influences of MSC Infused via Different Ways and Different Time on Anti-tumor Activities of CIK/NK cells from Umbilical Cord Blood in K562 NOD/SCID Mice 黎阳 吴燕峰 王潇娉 黄绍良 方建培 黄科 魏菁 中山大学孙逸仙纪念医院儿科 drliyang@126.com Background Bone marrow mesenchymal stem cells (MSC) has a unique immunological characteristics： Low expression of classⅠMHC molecules Do not express classⅡMHC molecules Did not express Fas ligand and co-stimulatory molecules Therefore, MSC is survival possible long-term in the host body, even can be survival long-term in heterogeneity host. Background Cytokine-induced killer(CIK) cells are heterogeneous group of cells that obtained from co-cultured mononuclear cells of the human peripheral blood, cord blood or bone marrow with stimulation of variety cytokines such as IL-2, IFN-γ，Anti-CD3 in vitro after a period of time. CIK cells expressing CD3 and CD56.  Background Studies have showed that MSC has a negative regulatory role, whether or not its can effect the anti-tumor activity of subsequent infused killer cells in vivo is worth being further studied. Background Because CD3+CD56+ CIK cells derived from CD3+CD56- T cells, MSC may also inhibit anti-tumor activity of CD3+CD56+ CIK cells. Objectives To explore the influences of MSC infused via different ways and at different time on anti-tumor activities of CIK/NK cells from umbilical cord blood in K562 NOD/SCID mice.   Experimental priniple： MSC CIK/NK cells Inhibition infusion infusion K562 NOD/SCID mice Shorten survival Increase in tumor burden Experimental groups： NOD/SCID mice MSC CIK/NK Group A at the same time (caudal vein) (caudal vein) Group B Delayed 48h MSC CIK/NK (caudal vein) (caudal vein) Group D Group C MSC CIK/NK (marrow cavity) (caudal vein) at the same time Delayed 48h MSC