GeneexpressionofABC-transportersinthefungal.PDF

Gene expression of ABC-transporters in the fungal biocontrol agent Clonostachys rosea in response to anti-fungal metabolites from Pseudomonas chlororaphis Jinhui Wang Independent project in biology-Master’s thesis, 30 hp, EX0564 Swedish University of Agricultural Sciences Department of Forest Mycology and Plant Pathology Uppsala 2012 Plant Biology-Master’s programme Gene expression of ABC-transporters in the fungal biocontrol agent Clonostachys rosea in response to anti-fungal metabolites from Pseudomonas chlororaphis Supervisor: Associate Prof. Magnus Karlsson Department of Forest Mycology and Plant Pathology Swedish University of Agricultural Sciences (SLU) Co-supervisor: Prof. Dan Funck Jensen Department of Forest Mycology and Plant Pathology Swedish University of Agricultural Sciences (SLU) Dr. Margareta Hökeberg Department of Forest Mycology and Plant Pathology Swedish University of Agricultural Sciences (SLU) Examiner: Associate Prof. Malin Elfstrand Department of Forest Mycology and Plant Pathology Swedish University of Agricultural Sciences (SLU) Swedish University of Agricultural Sciences Department of Forest Mycology and Plant Pathology Master Thesis in Plant Biology Course code: EX0564 30 hp D level Uppsala 2012 http://stud.epsilon.slu Abstract: Clonostachys rosea strain IK726 and Pseudomonas chlororaphis strain MA342 are two active biological control microbes against many seed and soil-borne plant diseases. A combination mixture between C. rosea IK726 and P. chlororaphis MA342 may provide additive or synergistic effects on the biocontrol ability of these microbes, due to complementing modes of action. However, it is not known if C. rosea IK726 can tolerate the antifungal metabolites produced by P. chlororaphis MA342. In the current study, a dual culture assay and mycelial dry