聚乙二醇基质地亲水整体柱研究.pdfVIP

度，来提升整体柱亲水的性能。在该部分中本体系采用自制的小型 低温柱温箱，实现了温度从室温到负二十度的可控调节。实验中我 们系统地评价了纯PEG整体柱从室温至负二十度条件下，对核苷类、 苯酚类、苯甲酸类、酰胺类、碱基类等极性化合物分离保留行为。 结果很好的证明了温度对亲水行为调节具有很好的效果，通过调节 温度使得PEG整体柱亲水性得到了提升。 本论文结果为亲水材料亲水性提升研究进行了有益探索，为亲 水分离模式的发展提供了方向。 关键词：反相色谱；亲水色谱；聚乙二醇；葡聚糖；硫酸葡聚糖 万方数据 Abstract In interaction recentlydecades，hydrophilicliquid arival toreversion (HILIC)，astechnique phaseliquid (RPLC)for and separatingpolarhydrophilicanalytes，hasincreasingly receivedattentionofthe isstil l exista researchers．However’there large in to mainreasonsasfollows： gap applicationcomparingRPLC，the ofthe materialsis firstly,thehydrophilicability separation quite and ofthe is insufficient，separationanalysisobjectslimited；secondly, the of materialis results varietyhydrophilic thoroughlydeficient，which inlessavailabletothe to additiontheabove option researchers；In reasonhinderits isthatthe hasless factors，the developmentinvestigator ofHILICthanRPLCthe knowledge and columnofHILICistoo and HILICstillhasalotof expensive．Therefore，developingpromoting to besolved． problems The focuseson thecolumn’S investigator promoting hydrophilic andhas beenstudiedintwo