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关键词 法夫酵母,虾青素,低聚果糖,联产发酵,分离纯化
II
Simultaneous production of astaxanthin and
fructooligosaccharide by Phaffia rhodozyma JMU-MVP14
strain
Abstract
Phaffia rhodozyma which is considered as one of the most important sources of nature
astaxathin is easly to culture and grow fastly in many media. In addition, Phaffia rhodozyma was
revealed to synthesize functional fructooligosaccharide. The simutaneous production of both
fructooligosaccharide and astaxanthin which would enhance the practice value of Phaffia
rhodozyma is considered of great significance.
This thesis which was expected to provide the foundation for further development and
utilization of the astaxanthin high yielding Phaffia rhodozyma JMU-MVP14, was performed on
the identification of fructooligosaccharide composition, the investigation of the regulation of
fructooligosaccharide synthesis, the optimization of the process for the production of
fructooligosaccharide, the exploration of coproduction of astaxthin and fructooligosaccharide in
7 L fermentor and purification of fructooligosaccharide from the cultured broth of this yeast
strain. The results are showed as the followings:
(1) The condition for runing the high performance liquid chromatography (HPLC) was
optimzed to show the mobile phase consisting of 72% acetonitrile and 22% water was proper for
the determination of the carbonhydrate in the broth of Phaffia rhodozyma JMU-MVP14 by the
HPLC method in a isocratic elution procedure.
(2) There were six kinds of carbonhydrate in the broth of Phaffia rhodozyma JMU-MVP14
detected by HPLC-RID and
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