法夫酵母JMU-MVP14联产发酵虾青素及低聚果糖的地研究.pdf

关键词 法夫酵母，虾青素，低聚果糖，联产发酵，分离纯化 II Simultaneous production of astaxanthin and fructooligosaccharide by Phaffia rhodozyma JMU-MVP14 strain Abstract Phaffia rhodozyma which is considered as one of the most important sources of nature astaxathin is easly to culture and grow fastly in many media. In addition, Phaffia rhodozyma was revealed to synthesize functional fructooligosaccharide. The simutaneous production of both fructooligosaccharide and astaxanthin which would enhance the practice value of Phaffia rhodozyma is considered of great significance. This thesis which was expected to provide the foundation for further development and utilization of the astaxanthin high yielding Phaffia rhodozyma JMU-MVP14, was performed on the identification of fructooligosaccharide composition, the investigation of the regulation of fructooligosaccharide synthesis, the optimization of the process for the production of fructooligosaccharide, the exploration of coproduction of astaxthin and fructooligosaccharide in 7 L fermentor and purification of fructooligosaccharide from the cultured broth of this yeast strain. The results are showed as the followings: (1) The condition for runing the high performance liquid chromatography (HPLC) was optimzed to show the mobile phase consisting of 72% acetonitrile and 22% water was proper for the determination of the carbonhydrate in the broth of Phaffia rhodozyma JMU-MVP14 by the HPLC method in a isocratic elution procedure. (2) There were six kinds of carbonhydrate in the broth of Phaffia rhodozyma JMU-MVP14 detected by HPLC-RID and