Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus.pdfVIP
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Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus.pdf
Availab4eontjNeatwww.sciencedirect.coro
∞
JournalofIntegrativeAgriculture
ScienceDirect N
2014,13(11):2452—2459 ovember2014
BiochemicalMechanism ofChlOrantranIliprOle Resistance in the
DiamondbackMoth.P|utel|a ostellaLinnaeus
HU Zhen—di。
, FENGXia,,L1NQing.sheng,,CHENHuan.yu,LIZhen—yu,,YINFei,,LIANG
PeiandGAO Xi.wu
InstituteofPlantProtectionGuangdongAcademyofAgricuhuralSciences,Guangzhou510640,P.R.China
GuangdongProvincialKeyLaboratoryofHighTechnologyforPlantProtection,Guangzhou510640.P.R.China
DepartmentofEntomology,ChinaAgriculturalUniversity,Beijing100193,P.R.China
Abstract
Theinsecticidechlorantraniliproleexhibitsgoodefficacyandplaysanimportantroleincontrollingthediamondbackmoth,
P tel/axylostellaLinnaeus.Howeverresistancetochloranrtaniliprolehasbeenobservedrecentlyinsomefieldpopulations.At
presentstudy,diamondbackmothswithresistancetochlorantraniliprole(resistantratio(RR)was82.18)forbiochemicalassays
wereselected.Theassayswereperformedtodeterminepotentialresistancemechanisms.Theresultsshowedthattheselected
resistantmoths(GDLZ-R)andsusceptiblemothcouldbesynergizedbyknownmetabolicinhibitorssuchaspiperonylbutoxide
(PBO),triphenylphosphate(TPP)anddiethyl-maleate(DEM)atdifferentlevels(1.68—5.50一foldand2.20—2.89一fold,respectively),
andDEM showedthemaximumsynergisminbothstrains.Inenzymesassays,ahighlevelofglutathione—S-transferase(GST)
wasobservedintheresistantmoth,incontrast,mothsthataresusceptibletotheinsecticidehadonly 1/3theGSTactivityofthe
resistantmoths.Theanalysisofshort—temr exposureofchlorantraniliproleonbiochemicalresponseintheresistantsrtainalso
showedthatGSTactivitywassignificantlyelevatedafterexposuretoasub-lethalconcentr
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