NaCl-lnduced Changes of Ion Fluxes in Roots of Transgenic Bacillus thuringiensis （Bt） Cotton （Gossypium hirsutum L.）.pdfVIP

Availableonlineat、^，、^^Ⅳ．sciencedirect．com JournalofIntegrativeAgriculture L_l”SScciieenncceeDiinrect M 2013，12(3)：436—444 arch20l3 NaCI—InducedChangesofIonFluxesinRootsofTransgenicBacillus thuringiensis(Bt)Cotton(GossypiumhirsutumL．) LIMao—ying，LIFang-jun，YUEYue—sen，TIANXiao—li，LIZhao—huandDUANLiu—sheng StateKeyLaboratoryofPlantPhysiologyandBiochemistryMinistryofEducation~EngineeringResearchCenterofPlantGrowthRegulator MinistryofEducation~Collegeof AgronomyandBiotechnology，ChinaAgriculturalUniversity，Bejiing100193，PRChina Abstract Bacillusthuringiensis(Bt)cottonisgrownworldwide，includinginsalinesoils，buttheeffectofsalinityonionfluxesof Btcottonremainsunknown．ResponsesoftwotransgenicBtcottongenotypes(SGK321and293171andtheircorresponding receptors，Shiyuan321(SY321)andJihe321(J321)，to150mmolL～NaC1stresswerestudiedinagrowthchamber．Theroot dryweightofSGK321and29317underNaC1treatmentwasdecreasedbv30and31％，respectively．However，their correspondingreceptorcultivarsSY321andJ321werelessaffected(19and24％，respectively)．Therootlengthand surfaceareaoftheBtcultivarsweresignificantlydecreasedrelativetotheirreceptorsundersaltstress．NaCltreatment significantlyincreasedCrylAcmRNA transcriptlevelsinSGK321and29317butdidnotaffectBtproteincontentin】eaves orrootsofeithercultivarat1and7dafterNaCltreatment．FluxesofNa．K+．andH+inrootswereinvestigatedusingthe scanningion—selectiveelectrodetechnique．BothmeanK e御uxrateandtransientK+efnuxoftheBtcultivarsincreased four—foldcomparedtotheircorrespondingreceptorswhenexposedtosalinitystress．Therewerenosignificantdifferences inNa em uxbetweenBtandnon．Btcottons．Furthermore．theNa+contentsinrootsand leavesofallgenotypes dramaticallyincreasedundersaltstress．whereasK contentsdecreased．OurresultssuggestedthatBtcottoncultivars aremoresensitiveto saltstressthantheirreceptorgenotypes． Ke