毕业设计（论文）_微波协助枸杞色素提取的优化研究.docVIP

目 录 摘 要 1 Abstract 2 前 言 3 第一章 引言 4 1.1 概述 4 1.2 枸杞的化学成分和药理功效 4 1.2.1枸杞的化学成分 4 1.2.2枸杞的药理功效 4 1.3 本课题的研究内容及意义 5 第二章 实验材料与方法 6 2.1 实验材料 6 2.1.1实验仪器和设备 6 2.1.2实验材料与试剂 6 2.2 实验方法 7 2.2.1枸杞色素提取流程 7 2.2.2枸杞色素的提取研究 7 2.3 枸杞色素的纯化方法 10 2.3.1色素的液-液萃取纯化 10 第三章 结果分析与讨论 11 3.1 枸杞色素最大吸收波长的测定 11 3.2有机溶剂浸提实验 12 3.2.1浸提溶剂的选择 12 3.2.2温度对浸提效果的影响 13 3.2.3时间对浸提效果的影响 13 3.2.4浸提次数对浸提效果的影响 14 3.2.5液料比对浸提效果的影响 14 3.2.6正交试验 14 3.2.7枸杞色素的浸提率 15 3.3 微波萃取试验 16 3.3.1微波火力的选择 16 3.3.2微波处理时间的选择 16 3.3.3液料比的选择 17 3.3.4微波萃取次数的选择 17 3.3.5微波萃取的正交试验 17 3.3.6枸杞色素的浸提率 18 结 论 19 参考文献 20 致 谢 21 摘 要 枸杞色素是一种天然食用色素，有较高的营养价值，被广泛应用于食品制作中。随着对合成色素安全性疑虑的加深，天然食用色素的开发和利用成为人们关注的焦点，这也促进了从枸杞中提取色素的研究。 本文以宁夏枸杞为原料，研究枸杞色素提取的优化条件。实验采用有机溶剂浸提枸杞子中的色素，通过单因素和正交实验确定了枸杞色素最适的提取工艺参数，即：用石油醚-丙酮（4:1 V:V）作浸提剂，液料比为3:1（VW），在50℃下浸提4次，每次1.5h，浸提率可达91.%；同时也通过单因素和正交实验确定了微波辅助萃取枸杞色素的最佳工艺条件：萃取次数为3次，微波火力为80%，每次萃取时间为20s，液料比为3:1（VW），浸提率为89.5%。 关键词：枸杞；色素；提取；正交试验 ABTRACT Pigment of Fructus Lycii is one kind of natural food colorings, which has higher nutritional value, is widely used in food production. It promotes the research of extracting pigment from the Fructus Lycii by heavy doubts about the safety of synthetic pigments, and people focus on the development of natural food coloring and use. The paper used Fructus Lycii dregs which was produced in Ningxia as raw material to study excellent extraction efficiency of Fructus lycii Pigment by organic solvent and microwave extractions.The optimum extracting conditions were got.The condition were temperature 50℃，for 1.5hours every times,ratio of liquid to material was 3:1(V/W),four times with petroleum ether-acetone(4:1 V:V).Under these conditions,the extraction rate was 91.4%.The result showed that by single factor experiments and orthogonal test : the microwave extractions optimum extraction condition was microwave power 80%,for 20s every times,ratio of liquid to material was 3:1(V/W) three times,the extraction rate was 89.5%. Keywords: Fructus lycii;Pigment;Extraction;Orthogonaltes 前