不同处理方法对本小果树HASKAOP组培苗生根的影响.pdfVIP

摘 要 日本北海道特产小果树HASKAOP (Lonicera caerulea L. var. emphyllacaryx Nakai)原为野生，属于木本植物，具有较高的营养价值、药用价值和经济价值。在 组织培养中，芽容易诱导和增殖，根却难以诱导。诱导生根是利用组织培养技术进 行微繁殖的关键。本试验对小果树试管苗的生根处理方法及生根培养条件进行了初 步探索，主要研究结果如下： 1.IBA 对小果树的生根有明显促进作用，NAA 和IAA 对其生根促进作用不明 显。0.4mg/L 的IBA 是适宜小果树生根的最佳浓度。 2．在两步生根过程中，适宜小果树预处理生根诱导的IBA 浓度是80mg/L，适 宜小果树预处理生根的IBA 天数为11d。 3．不同浓度IBA 浸渍对小果树生根有影响，最佳生根是在100mg/LIBA 中浸 渍15min。 4 ．适宜小果树生根的基本培养基为1/2MS 培养基。 5．适合小果树生根的暗培养时间为5d。 6．适合小果树生根的AC浓度为3g/L。 7．适合小果树生根的蔗糖浓度是20~30g/L。 8．适合小果树移栽的基质为1/3 蛭石+2/3 田园土。 关键词：小果树；不同处理方法；生根 Effects of Different Treatment Methods on Rooting of HASKAOP Abstract The Japanese Hokkaido special product small fruit tree (Lonicera caerulea L. var. emphyllacaryx Nakai) is originally wild fruit tree, and belongs woody plant with high nutritional, medicinal and economic values. Shoot induction and proliferation is easy to root inducement of tissue culture. Rooting is the key of using tissue culture for micro-propagation. The test tube of small fruit tree rooting and rooting treatment conditions were explored, the main results of this research were as follows: 1. IBA had obvious effect on the small fruit trees rooting. NAA and IAA was not obvious. The optimal concentration on rooting of small fruit trees was 0.4 mg/L IBA. 2. In the two-step process of rooting, the optimum IBA concernment for pretreatment rooting induction of small fruit tree was 80mg/L. The optimum IBA time for pretreatment rooting of Small fruit trees was 11 days. 3. The different sucrose concentrations of IBA dipping had effect on rooting of small fruit tree, the optimal condition on rooting was in 100mg/LIBAdipping for 15min. 4. The optimal basic media for rooting of s