Caco-2细胞体外吸收模型的建立及评估.pdf

SouthMed ·548· 南方医科大学学报(J Univ) 2009；29(3) Caco一2细胞体外吸收模型的建立及评估 查龙应，罗海吉，邓红，楚心唯(南方医科大学公共卫生与热带医学学院营养与食品卫生学系，广东广州510515) 摘要：目的建立Caco．2细胞体外吸收模型并对其进行评估。方法将细胞接种在Snapweil转运培养槽的微孔滤膜上。 进行体外培养。采用细胞形态学、跨膜电阻值、甘露醇透过率和碱性磷酸酶活性等指标对细胞模型进行检测。结果培养 21 性磷酸酶活性显著高于基底侧酶活性。结论在本实验室条件下构建的Caco一2细胞模型在形态上与小肠上皮细胞相 似．细胞已产生极性．可作为小肠吸收的体外模型。 关键词：Caco．2细胞；吸收；跨膜电阻；甘露醇透过率；碱性磷酸酶 中图分类号：02．33 文献标识码：A 文章编号：1673—4254(2009)03-0548—03 invitro model EstablishmentandassessmentofCaco一2cell absorption ZHA Xin-wei Long-ying，LUOHai-ji，DENGHong，CHU and Medical ofNutritionandFood ofPublicHealthTropicalMedicine，Southern Department Hygiene，School 510515，China ToestablishandassesstheCaco．2eellirtvitro model．MethodsCaco-2cellswerecultured Abstract：0bjective absorption cell onthe filtersfixedin chamber．ne electricalresistance，mannitol millipore Snapwelltransport morphology，transepithelial rateandalkaline activitiesweremonitoredculture．ResulIsAtier21daysofinvitro emux phosphatase during culture， thecells．The electricalresistancereacha stable formationof iunctionwasobservedbetween tight transepithelial relatively valueof620_+47Q·cm2．themannitoleffiUXratewaslowerthan0．3％·h1·cm。2，andthealkaline inthe phosphataseactivity sidewas thanthatinthebasolateralside．ConclusionTheestablishedCaco-2eellmodelshowssimilar apical significantlyhigher tointestinal cellswithformation Canbeusedasaninvitromodelfor stud