Effect of Silicon, Titanium, and Zirconium Ion Implantation on NiTi Biocompatibility.pdfVIP

Hindawi Publishing Corporation Advances in Materials Science and Engineering Volume 2012, Article ID 706094, 16 pages doi:10.1155/2012/706094 Research Article Effect of Silicon, Titanium, and Zirconium Ion Implantation on NiTi Biocompatibility L. L. Meisner,1 A. I. Lotkov,1 V. A. Matveeva,2 L. V. Artemieva,2 S. N. Meisner,1 and A. L. Matveev2 1 Institute of Strength Physics and Materials Science, SB RAS, Akademichesky 2/4, Tomsk 634021, Russia 2 Institute of Chemical Biology and Fundamental Medicine, SB RAS, Lavrent’eva 8, Novosibirsk 630090, Russia Correspondence should be addressed to L. L. Meisner, meisner2l@ Received 31 March 2011; Accepted 19 September 2011 Academic Editor: W. Ensinger Copyright © 2012 L. L. Meisner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The objective of the work was to study the effect of high-dose ion implantation (HDII) of NiTi surface layers with Si Ti, or Zr, on the NiTi biocompatibility. The biocompatibility was judged from the intensity and peculiarities of proliferation of mesenchymal stem cells (MSCs) on the NiTi specimen surfaces treated by special mechanical, electrochemical, and HDII methods and differing in chemical composition, morphology, and roughness. It is shown that the ion-implanted NiTi specimens are nontoxic to rat MSCs. When cultivated with the test materials or on their surfaces, the MSCs retain the viability, adhesion, morphology, and capability for proliferation in vitro, as evidenced by cell counting in a Goryaev chamber, MTT test, flow cytometry, and light and fluorescence microscopy. The unimplanted NiTi specimens fail to stimulate MSC prolifer