拔节至成熟期遮荫对小麦籽粒高分子量麦谷蛋白亚基积累及GMP含量的影响.pdfVIP

中国农业科学 2009,42(10):3451-3458 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.10.009 拔节至成熟期遮荫对小麦籽粒高分子量麦谷蛋白亚基积累 及GMP含量的影响 伯 云，李华伟，牟会荣，蔡 剑，周 琴，戴廷波，曹卫星，姜 东 （南京农业大学农学院/农业部南方作物生理生态重点开放实验室/江苏省信息农业高技术研究重点实验室，南京 210095 ） 摘要：【目的】高分子量麦谷蛋白亚基（HMW-GS）和麦谷蛋白大聚合体（GMP）含量是反映小麦籽粒品质的重 要性状，本试验研究拔节到成熟期遮荫对2个含相同HMW-GS亚基类型（7+8、2+12）小麦品种胚乳HMW-GS积累动 态和GMP含量的影响。【方法】采用SDS电泳和切胶比色的方法，对遮荫条件下小麦籽粒中HMW-GS亚基进行 定量分析。【结果】遮荫使HMW-GS的起始形成时间提早，18%和25%重度遮荫处理缩短各亚基和总亚基快速积累期， 并降低灌浆后期积累速度，成熟期单粒总 HMW-GS 积累量低于对照，但成熟期籽粒 HMW-GS 和 GMP 含量高于对照。 轻度遮荫（10%）延长了籽粒HMW-GS快速积累期，亚基积累量和含量均高于对照。【结论】遮荫处理影响小麦籽粒 HMW-GS积累，但其效应与遮荫强度有关。 关键词：小麦；遮荫；高分子量谷蛋白亚基（HMW-GS）；谷蛋白大聚合体（GMP） Effects of Shading From Jointing Stage to Maturity Stage on High Molecular Weight Glutenin Subunits Accumulation and Glutenin Macropolymer Content in Wheat Grain BO Yun, LI Hua-wei, MU Hui-rong, CAI Jian, ZHOU Qin, DAI Ting-bo, CAO Wei-xing, JIANG Dong (Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Jiangsu Hi-Tech Key Laboratory for Information Agriculture, College of Agronomy, Nanjing Agricultural University, Nanjing 210095) Abstract: 【Objective 】 Accumulations of high molecular weight glutenin subunits (HMW-GS) and glutenin macropolymer (GMP) play key roles in determining grain quality in wheat (Triticum aestivum L.). Two wheat cultivars owning the same HMW-GS type (7+8 and 2+12) were selected to study the effects of shading from jointing to maturity on HMW-GS accumulation and GMP content in grain. 【Method 】 HMW-GS was firstly separated using the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS), and each HMW-GS subunit band in the gel was cut and extracted. Content of each HMW-GS