复方益母生化散对奶牛子宫内膜细胞CYP450表达和免疫功能的影响.pdfVIP

中国农业科学 2010,43(6):1248-1254 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.06.019 复方益母生化散对奶牛子宫内膜细胞CYP450表达 和免疫功能的影响 杜金梁，秦建华，褚景生，徐丽娜，马玉忠 （河北农业大学动物科技学院，河北保定 071001 ） 摘要：【目的】探讨复方益母生化散对奶牛子宫内膜炎的作用机理。【方法】体外实验：分离奶牛子宫内膜细 胞，细菌脂多糖（LPS）诱导子宫内膜细胞炎症模型，复方益母生化散处理子宫内膜炎症细胞48 h、72 h，Western blotting法检测CYP450的表达；体内实验：复方益母生化散栓剂治疗患有子宫内膜炎的奶牛，检测血清中IgG、 IgA 含量的变化。【结果】复方益母生化散处理后，子宫内膜炎症细胞 CYP450 的表达随复方益母生化散剂量的增 加而呈升高的趋势，血清中 IgG、IgA 的含量与对照组相比明显升高。【结论】2 000 µg·mL-1 的复方益母生化散作 用奶牛子宫内膜炎症细胞48 h，CYP450的表达最明显。 关键词：奶牛；子宫内膜细胞；CYP450；复方益母生化散；免疫功能 Effects of Yimu Shenghuasan on Cytochrome P450 of Endometrial Cells and Immune Function in Dairy Cows DU Jin-liang, QIN Jian-hua, CHU Jing-sheng, XU Li-na, MA Yu-zhong (College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, Hebei) Abstract: 【Objective 】 The aim of the study is to investigate the acting mechanism of Yimu Shenghuasan on dairy cow endometrial cells. 【Method 】In vitro experiment, the primary cultured endometrial cells in cows were isolated and the inflammatory models were made by lipopolysaccharide (LPS) induction. The inflammatory cells were treated with gradient concentrations of herbal medicine prescription Yimu Shenghuasan for 48h and 72h, then the Western blot was used to detect the expression of CYP450. In vivo experiment, the suppository Yimu Shenghuasan was put into the endometritis dairy cows to detect the content of IgG and IgA in sera. 【Results 】 The expression of cytochrome P450 in dairy cow endometrial cells increased gradually by Western blot; compared with the control group, the content of IgG, IgA