根瘤菌基因组内简单重复序列的分析.pdfVIP

中国农业科学 2008,41(10):2992-2998 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.10.011 根瘤菌基因组内简单重复序列的分析 高亚梅，韩毅强，汤 辉，孙东梅，王彦杰，王伟东 （黑龙江八一农垦大学生命科学技术学院，黑龙江大庆 163319 ） 摘要：【目的】分析根瘤菌基因组中的简单重复序列（simple sequence repeats，SSRs），为其在根瘤菌遗 传多样性研究中的应用提供有益的信息。【方法】利用公共的微生物串联重复序列数据库资源，对已测序的3种根 瘤菌基因组中 SSRs 的结构类型、分布、丰度等进行系统的比较分析。【结果】大豆慢生根瘤菌（ Bradyrhizobium japonicum ）、百脉根根瘤菌（Mesorhizobium loti ）和苜蓿中华根瘤菌（Sinorhizobium meliloti ）基因组中的 SSRs 分别为 1 410 个、859 个和 638 个，3 种根瘤菌基因组中长重复的四、五、六核苷酸基序更为丰富，变异性 更高。数目最少的为单碱基重复。【结论】3种根瘤菌的SSR在结构类型和分布规律上均具有一定的相似性。 关键词：根瘤菌；基因组；微卫星序列 Analysis of Simple Sequence Repeats in Rhizobium Genomes GAO Ya-mei, HAN Yi-qiang, TANG Hui, SUN Dong-mei, WANG Yan-jie, WANG Wei-dong (College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, Heilongjiang) Abstract: 【Objective 】Simple sequence repeats (SSRS)or microsatellites, as genetic markers, are ubiquitous in genomes of various organisms. The analysis of SSR in rhizobia genome has provided useful information for a variety of applications in population genetics of rhizobia. 【Method 】This study examined the SSRs in completely sequenced Bradyrhizobium japonicum, Mesorhizobium loti and Sinorhizobium meliloti genomes in the Microorganisms Tandem Repeats Database. The occurrences, relative abundance, relative density, most common and longest SSRs in the three species were analyzed and compared with each other. 【Result 】 The results revealed that the SSRs number in B. japonicum, M. loti and S. meliloti genomes were 1 410, 859 and 638, respectively. In the three genomes, t