花后干湿交替灌溉对水稻强、弱势粒蛋白质表达的影响.pdfVIP

中国农业科学 2013,46(22):4665-4678 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.22.004 花后干湿交替灌溉对水稻强、弱势粒蛋白质表达的影响 陈婷婷，褚 光，华小龙，潘 燕，王志琴，杨建昌 （扬州大学农学院/江苏省作物遗传生理重点实验室，江苏扬州 225009 ） 摘要：【目的】进一步揭示干湿交替灌溉对水稻籽粒灌浆影响的机制。 【方法】两优培九（两系杂交籼稻） 和扬粳 4038 （粳稻）种植于土培池，自抽穗至成熟设置常规灌溉（conventional irrigation，CI）、轻干-湿交 替灌溉（alternate wetting and moderate drying irrigation，WMD）和重干-湿交替灌溉（alternate wetting and severe drying irrigation，WSD）3 种土壤水分处理，运用双向电泳技术测定水稻强、弱势粒蛋白质表达量。 【结果】与CI 相比，WMD 和WSD 对强势粒的灌浆速率和粒重无显著影响；WMD 显著增加了弱势粒的灌浆速率和粒 重，WSD 则显著降低了弱势粒的灌浆速率和粒重。WMD 和WSD 对强势粒蛋白质表达无显著影响，但WMD 上调了弱势 粒中丙酮酸磷酸双激酶、甘油醛-3-磷酸脱氢酶、5- 甲基四氢叶酸-同型高半胱氨酸甲基转移酶、S-腺苷甲硫氨酸 合酶、乙二醛酶I 和锰超氧化物歧化酶等蛋白质的表达，WSD 则下调了弱势粒中上述蛋白质的表达。WSD 还上调了 抑制信号传导和能量代谢等有关蛋白的表达。两品种结果趋势一致。 【结论】说明在WMD 或WSD 条件下，弱势粒 中多种与灌浆相关蛋白质表达的差异是其灌浆速率和粒重增加或降低的重要原因。 关键词：水稻；干湿交替灌溉；弱势粒；灌浆；蛋白质表达 Effect of Post-Anthesis Alternate Wetting and Drying Irrigation on Protein Expressions in Superior and Inferior Spikelets of Rice CHEN Ting-ting, CHU Guang, HUA Xiao-long, PAN Yan, WANG Zhi-qin, YANG Jian-chang (College of Agriculture, Yangzhou University/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou 225009, Jiangsu) Abstract: 【Objective 】This study aimed to understand the mechanism underlying the effect of alternate wetting and drying irrigation on grain filling of rice. 【Method 】Two rice cultivars, Liangyoupeijiu (two-line indica hybrids) and Yangjing 4038 (japonica ), were grown in cement tanks. Three irrigation regimes, conventional irrigation (CI), alternate wetting and moderate drying irrigation (WMD) and alternate wetting and severe drying irrigation (WSD), were imposed from full heading to maturity. The e