花期干旱胁迫对籼稻近等基因系水分和光合生理的影响.pdfVIP

中国农业科学 2013,46(7):1481-1491 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.07.019 花期干旱胁迫对籼稻近等基因系水分和光合生理的影响 杨永杰，张彩霞，宋 建，熊 杰，王 熹，章秀福，符冠富，陶龙兴 （中国水稻研究所/水稻生物学国家重点实验室, 杭州 310006 ） 摘要：【目的】利用一套表观性状显著差异的籼稻近等基因系，在研究花期干旱胁迫对育性影响的基础上， 研究其水分和光合生理响应过程。分析表观农艺性状改变对水稻水分和光合生理的影响和作用机制，分析表观农 艺性状、水分和光合生理与耐旱性之间的联系，以期揭示水稻花期耐旱性主效基因的生理性状指标。【方法】于主 茎见穗至此后的15 d 进行干旱处理，研究花期干旱胁迫对水稻近等基因系剑叶和根系生理活性的影响。【结果】 花期干旱胁迫下，各水稻近等基因系表现出不同的抗旱性，且耐旱性与表观农艺性状间并无明显规律可循，与正 常浇水条件下水稻本身的水分和光合特性也无显著相关性，但花期干旱胁迫条件下，水稻抗旱系数与剑叶含水率 和剑叶水势降幅的相关系数分别为0.614**和0.514**，与气孔导度降幅的相关系数为0.541**，表明花期干旱胁 迫下水稻的耐旱性与剑叶含水量、水势和气孔导度的降幅这3 个水分生理参数密切相关。此外，除Fv/Fm 的降幅 与抗旱系数的相关系数为 0.470*外，花期干旱胁迫下水稻光合生理指标改变值与耐旱性无显著相关性。【结论】 水稻花期干旱胁迫下的水分生理参数变化值可作为筛选耐旱水稻品种的指标。 关键词：水稻；近等基因系；花期干旱胁迫；生理活性 Effects of Drought Stress on Water and Photosynthetic Physiology Activities of Near-Isogenic Indica Rice Lines at Flowering Stage YANG Yong-j ie, ZHANG Cai-xia, SONG Jian, XIONG Jie, WANG Xi, ZHANG Xiu-fu, FU Guan-fu, TAO Long-xing (China National Rice Research Institute/State Key Laboratory of Rice Biology, Hangzhou 310006) Abstract: 【Objective 】An indica near-isogenic rice lines were treated with drought stress at flowering stage to study water and photosynthetic physiology changes of flag leaf. By analyzing the relationships among agronomic phenotype characters, water and photosynthetic physiology changes and fertility of rice, the authors try to accumulate data for drought tolerance evaluation of rice. 【Method 】The rice materials were suffered with drought stress begin from heading of main shoot for 15 days, water and photosynthetic physiology parameters were measured after treatment. 【Result 】The results showed that the indica near-isogenic rice