苗期水稻叶片发育进程的差异蛋白质组学分析.pdfVIP

中国农业科学 2008,41(11):3831-3837 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.11.052 苗期水稻叶片发育进程的差异蛋白质组学分析 1,2 2 2 邵彩虹 ，王经源 ，林文雄 1 2 （江西省农业科学院土壤肥料与资源环境研究所，南昌 330200 ； 福建农林大学农业生态研究所，福州 350002 ） 摘要： 【目的】从蛋白质组学角度对杂交水稻汕优 63 苗期的叶片蛋白质组变化进行研究，以揭示水稻苗期 叶片蛋白质组的表达差异。【方法】蛋白质点经双向电泳得到分离，并采用Imagemaster 2D Elite 5.0软件对所 得到的双向电泳图谱进行分析比较。【结果】经图谱分析共得到差异蛋白质点41个，其中三叶期后新增蛋白质点 17 个,包括 9 个仅在三叶期出现特异的特异蛋白质点，13 个蛋白质点表达丰度先逐渐增强随后减弱或在消失，其 它11个蛋白质点中，自二叶期开始表达丰度呈现下降的蛋白质点有3 个，保持上升的有6个，不规律出现的有 2 个；其中的15 个蛋白质点经质谱分析得到鉴定。【结论】蛋白质是细胞功能执行者，受细胞功能或细胞环境变化 的影响，因此,上述蛋白质发生变化的原因可能与水稻苗期分蘖发生有关系。 关键词：水稻；分蘖；蛋白质组学；差异蛋白 Differential Proteomics Analysis of Leaf Development at Rice (Oryza sativa) Seedling Stage SHAO Cai-hong, WANG Jing-yuan, LIN Wen-xiong 1 ( Soil and Fertilizer Resources and Environment Institue, Jiangxi Academy of Agricultural Sciences, Nanchang 330200 ; 2Agroecological Institute, Fujian Agriculture and Forestry University, Fuzhou 35000) Abstract: 【Objective 】In order to understand the function of differential expression proteins in leaves of seedlings, the proteins of leaves at the seedling stage of hybrid Shanyou63 were studied by using approach of plant proteomics. 【Method 】 Proteins were isolated with 2-DE and then analyzed with Imagemaster 2D Elite 5.0. 【Result 】 The result showed that the number of detected differential expression protein spots was 41, among which there were 17 new protein spots that appeared after the 3-leaf stages including 9 special protein spots which only appe