基因表达分析稻瘟菌7个Rho GTP酶的关系.pdfVIP

中国农业科学 2006,39(11):2237-2242 Scientia Agricultura Sinica 基因表达分析稻瘟菌7个Rho GTP酶的关系 郑 武，陈继圣，郑士琴，刘文德，陈 晓，周 洁，鲁国东，王宗华 （福建农林大学生物农药与化学生物学教育部重点实验室，福建农林大学功能基因组学研究中心，福州 350002 ） 摘要：【目的】Rho GTP酶是Ras超家族小G蛋白最主要成员。不同生物均含多种Rho GTP酶，相互之间存在 密切关系，并共同参与调控多个信号转导途径。稻瘟菌基因组中存在7个Rho GTP酶，分析它们相互关系意义甚 大。【方法】通过 RT-PCR 分析了MgCdc42 不同状态下以及 MgRho3 插入失活状态下其它 Rho GTP 酶基因的表达变 化。【结果】结果表明 MgCdc42 可能处于MgRho1、MgRho2 和MgRhoX 上游，对这些蛋白起正调控作用，对MgRac1 和MgRho4 起着负调控作用；MgRho3 对MgRho1 和MgCdc42 的表达起正调控，对MgRac1 和MgRho4 的表达则起负调 控作用；MgRho3 与MgCdc42 间关系复杂。【结论】稻瘟菌 Rho GTP 酶信号途径复杂，不同 Rho GTP 酶间存在相互 调控关系，这将有助于进一步了解稻瘟菌等丝状真菌Rho GTP酶之间的相互关系。 关键词：稻瘟菌；RhoGTP 酶；调控机制 Relationship Among Seven Rho GTPases in Magnaporthe griesea Revealed by Gene Expression Analysis ZHENG Wu, CHEN Ji-sheng, ZHENG Shi-qin, LIU Wen-de, CHEN Xiao, ZHOU Jie, LU Guo-dong, WANG Zong-hua (The Ministry of Education Key Laboratory of Bio-Pesticide and Chemistry Biology, Functional Genomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002) Abstract: 【Objective 】The Rho GTPases are major members of the Ras superfamily proteins. It is well known that there are several Rho GTPases interacting with each other and regulating several signal transduction pathways in eukaryotes. Genome analysis showed that there are seven putative Rho GTPases in Magnaporthe grisea, and it is essential to study the relationship among these Rho GTPases. 【Method 】RT-PCR was used to analyze expression patterns of genes encoding these Rho GTPases in different MgCdc42 and MgRho3 mutants. 【Result 】It was found that MgCdc42 positively controlled expression MgRho1 , MgRho2 and MgRhoX and negatively regulated expression MgRac1 and MgRho4 ; however MgRho3