利用三倍体胚乳遗传模型定位爆裂玉米膨爆特性QTL.pdfVIP

中国农业科学 2006,39(3):448-455 Scientia Agricultura Sinica 利用三倍体胚乳遗传模型定位爆裂玉米膨爆特性QTL 李玉玲，董永彬，崔党群，牛素贞，王延召，余永亮 （河南农业大学农学院，郑州 450002 ） 摘要：【目的】探讨爆裂玉米膨爆特性的分子遗传基础，为遗传改良和分子标记辅助选择提供有益信息。【方法】 以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建259个F 家系群体，利用SSR标记构建分子标记遗传 2 ﹕3 图谱，利用三倍体胚乳遗传模型和区间作图法对F2 ﹕3群体在两种环境条件下的膨爆特性进行了QTL定位和效应分析。 【结果】构建的爆裂玉米SSR遗传连锁图谱包含183个标记，覆盖玉米基因组1762.2 cM，标记间平均距离为9.63 cM。爆裂玉米的膨爆特性是由效应不等的多基因控制的数量性状，同时受环境条件的影响。两种环境条件下共检测 到 18 个爆花率 QTL，17 个膨化体积 QTL 和 21 个膨化倍数 QTL。单个 QTL 可解释的表型变异介于 4.4%～28.0%，可 解释的表型总变异为65.8%～96.6%，基因的作用方式为加性、部分显性、显性和超显性的QTL数目分别为2、1、4、 20 和 1、5、1、22，超显性在膨爆特性的遗传中起着最重要的作用。【结论】与以往膨爆特性以加性效应遗传为主 的研究结果不一致的主要原因在于三倍体胚乳模型对显性效应的细分和对显性效应较小QTL的显性效应的高估。 关键词：爆裂玉米；三倍体胚乳；数量性状位点；QTL定位 QTL Mapping of Popping Characteristics in Popcorn Using the Model of Trisomic Inheritance in the Endosperm LI Yu-ling, DONG Yong-bin, CUI Dang-qun, NIU Su-zhen, WANG Yan-zhao, YU Yong-liang (Agronomy College of Henan Agricultural University, Zhengzhou 450002) Abstract: 【Objective 】The objective of this study was to investigate the molecular genetic basis of popping characteristics in popcorn and provide useful message for their genetic improvement and MAS. 【Method 】Two hundred and fifty-nine F2:3 families, derived from the cross between Dan232, a dent corn inbred and N04, a popcorn inbred, were evaluated for popping characteristics under two environments. One hundred and eighty three pairs of SSR markers were used to construct the genetic map. Quantative trait loci (QTL) were identified and their effects were evaluated for PR (popping rate), PV (popping volume) and PF (popping fold), using triploid endosperm genetic model and interval mapping method. 【Result 】The