临床型乳房炎与正常奶牛血浆的差异蛋白质组研究.pdfVIP

中国农业科学 2008,41(7):2162-2167 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.07.039 临床型乳房炎与正常奶牛血浆的差异蛋白质组研究 1,2 1 1 1 杨永新 ，赵兴绪 ，张 勇 ，陶金忠 1 2 （甘肃农业大学动物医学院，兰州 730070 ； 安徽省农业科学院畜牧兽医研究所，合肥 230031 ） 摘要： 【目的】检测和分析临床型乳房炎与正常奶牛血浆中差异表达的蛋白质。【方法】用二维凝胶电泳分 离血浆蛋白，凝胶用硝酸银染色和考马斯亮蓝染色，PDQuest7.4软件自动检测分析差异表达蛋白斑点并经高效液 相色谱串联离子阱质谱鉴定。【结果】凝胶图谱分析后得到 3 个差异蛋白斑点，鉴定为 2 种蛋白质（结合珠蛋白 前体和乳腺球蛋白）。结合珠蛋白前体在临床型乳房炎奶牛血浆中表达量增加，可作为乳房炎诊断标记分子；而乳 腺球蛋白的表达量下调。【结论】临床型乳房炎与正常奶牛的血浆中蛋白质的表达存在差异，对差异表达蛋白质 的研究有利于探索机体的生理病理状态，可为疾病的诊断和治疗提供全面的信息。 关键词：奶牛；临床型乳房炎；血浆；差异表达蛋白质 Comparative Proteomics Analysis of Plasma Between Healthy Dairy Cows and Clinical Mastitic Cows 1,2 1 1 1 YANG Yong-xin , ZHAO Xing-xu , ZHANG Yong , TAO Jin-zhong 1 2 ( College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070; Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031) Abstract: 【Objective 】Differential proteomic expressions in plasma from healthy dairy cows and clinical mastitic cows was investigated. 【Method 】The current research presents the protein changes using two-dimensional gel electrophoresis (2-DE) after stained with silver nitrate and colloidal coomassie blue, differential expression proteins were detected by PDQuest 7.4 software and subjected to ion trap mass spectrometer equipped with a Surveyor HPLC system, and differential spots of protein were identified. 【Result 】 Three protei