Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge.pdfVIP
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Acta0ceano1.Sin.,2014,VbI.33,No.10,P94—104
DOI:1O.1007/s13131-014—0544-0
http://www.hyxb.org.cn
E—mail:hyxbe@263.net
Microbialcommunitystructureandnitrogenasegenediversiyt
ofsedimentfrom adeep.seahydrothermalventfieldonthe
SouthwestIndina Ridge
WUYuehong1~,CAOyi2~,WANGChunshengI,WU Min3,AHARONOren4,5,XUXuewei1
LaboratoryofMarineEcosystem andBiogeochem istry,SecondInstituteofOceanography,StateOceanic
Administration,Hangzhou310012,China
StateKeyLaboratory ofMicrobialResources,InstituteofM icrobiology,ChineseAcademyofSciences,
Beijing100101,China
0CollegeofLifeSciences,ZhejiangUniversity,Hangzhou310058,China
DepartmentofPlantandEnvironmentalSciences,theAlexanderSilbermanInstituteofLifeSciences,The
Hebrew UniversityofJerusalem,Jerusalem 91904,Israel
TheHebrew UniversiytofJerusalem.TheEdmondJ.SafraCampus,GivatRam,Jerusalem 91904,Israel
Received25March2013;accepted2November2013
~TheChineseSocietyofOceanographyandSpringer-VerlagBerlinHeidelberg2014
Abstract
Asedimentsamplewascollectedfrom adeep—seahydrothermalventfieldlocatedatadepthof2951m on
theSouthwestIndianRidge.Phylogeneticanalyseswereperformedontheprokaryoticcommunityusing
polymerasechainreaction (PCR)amplificationofthe16SrRNAandnf艘 genes.WithintheArchaea,the
dominantcloneswerefrom marinebenthicgroupE (MBGE)andmarinegroupI(MGI)belongingtothe
phylaEuryarchaeotaandThaumarchaeota.respectively.Morethanhalfofthebacterialclonesbelonged
totheProteobacteria,andmostfellwithintheGammaproteobacteria.Noepsi10npr0te0bacterialsequence
wasobserved.Additional phylaweredetectedincludingtheActinobacteria,Bacteroidetes。P nctomycetes.
Acidobacteria,Nitrospirae,Chloroflexi,Chlorobi,Chlamvdiae,Verrucomicrobia,andcandidatedivisions
0D1.0P11,W S3andTM6.confirmingtheirexistenceinhydrothermalventenvironments.Thedetectionof
n~/ngenesuggeststhatbiologicalnitrogenfixationmayoccurinthehydrothermalventfieldoftheSouth—
westIndianRidge.PhylogeneticanalysisindicatedthatonlyClustersIandIIINifH werepresent.Th
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