Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge.pdfVIP

Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge.pdf

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Acta0ceano1.Sin.,2014,VbI.33,No.10,P94—104 DOI:1O.1007/s13131-014—0544-0 http://www.hyxb.org.cn E—mail:hyxbe@263.net Microbialcommunitystructureandnitrogenasegenediversiyt ofsedimentfrom adeep.seahydrothermalventfieldonthe SouthwestIndina Ridge WUYuehong1~,CAOyi2~,WANGChunshengI,WU Min3,AHARONOren4,5,XUXuewei1 LaboratoryofMarineEcosystem andBiogeochem istry,SecondInstituteofOceanography,StateOceanic Administration,Hangzhou310012,China StateKeyLaboratory ofMicrobialResources,InstituteofM icrobiology,ChineseAcademyofSciences, Beijing100101,China 0CollegeofLifeSciences,ZhejiangUniversity,Hangzhou310058,China DepartmentofPlantandEnvironmentalSciences,theAlexanderSilbermanInstituteofLifeSciences,The Hebrew UniversityofJerusalem,Jerusalem 91904,Israel TheHebrew UniversiytofJerusalem.TheEdmondJ.SafraCampus,GivatRam,Jerusalem 91904,Israel Received25March2013;accepted2November2013 ~TheChineseSocietyofOceanographyandSpringer-VerlagBerlinHeidelberg2014 Abstract Asedimentsamplewascollectedfrom adeep—seahydrothermalventfieldlocatedatadepthof2951m on theSouthwestIndianRidge.Phylogeneticanalyseswereperformedontheprokaryoticcommunityusing polymerasechainreaction (PCR)amplificationofthe16SrRNAandnf艘 genes.WithintheArchaea,the dominantcloneswerefrom marinebenthicgroupE (MBGE)andmarinegroupI(MGI)belongingtothe phylaEuryarchaeotaandThaumarchaeota.respectively.Morethanhalfofthebacterialclonesbelonged totheProteobacteria,andmostfellwithintheGammaproteobacteria.Noepsi10npr0te0bacterialsequence wasobserved.Additional phylaweredetectedincludingtheActinobacteria,Bacteroidetes。P nctomycetes. Acidobacteria,Nitrospirae,Chloroflexi,Chlorobi,Chlamvdiae,Verrucomicrobia,andcandidatedivisions 0D1.0P11,W S3andTM6.confirmingtheirexistenceinhydrothermalventenvironments.Thedetectionof n~/ngenesuggeststhatbiologicalnitrogenfixationmayoccurinthehydrothermalventfieldoftheSouth— westIndianRidge.PhylogeneticanalysisindicatedthatonlyClustersIandIIINifH werepresent.Th

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