在白色杜洛克×二花脸F2资源群体中定位猪四肢骨骨骼长度和直径QTL.pdfVIP

中国农业科学 2009,42(4):1378-1385 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.04.031 在白色杜洛克×二花脸F 资源群体中定位猪四肢骨 2 骨骼长度和直径QTL 陈从英，郭源梅，杨 斌，任 军，黄路生 （江西农业大学动物生物技术国家重点实验室培育基地，南昌 330045 ） 摘要：【目的】通过全基因组扫描，鉴别影响猪四肢骨骨骼长度，股骨和肱骨的骨髓腔长度、骨髓腔直径以 及股骨骨壁厚度的数量性状位点（QTL）。【方法】在白色杜洛克×二花脸资源群体中测定132头240日龄阉割公猪 29 类四肢骨骨骼的长度、6 类四肢骨骨骼直径以及股骨和肱骨骨壁厚度、骨髓腔长度和骨髓腔直径等表型性状。 选择多态信息含量丰富并覆盖猪全基因组19条染色体的183个微卫星标记，采用最小二乘区间定位法进行猪全基 因组扫描，定位猪四肢骨骼各性状QTL。【结果】在39个表型性状中定位到14个基因组1%显著水平 QTL，14个基 因组 5%显著水平 QTL 和 47 个染色体 5%显著水平 QTL。除 SSC11 没有检测到 QTL 外，其它各染色体都存在影响四 肢骨骼QTL。【结论】定位75 个影响猪四肢骨骼性状QTL，在SSC7上57～59 cM 发现影响多种骨骼生长的QTL。 关键词：四肢骨骼；全基因组扫描；QTL定位；猪 Identification of QTL for Length and Diameter of Porcine Limb Bones in a White Duroc× Erhualian F2 Resource Population CHEN Cong-ying, GUO Yuan-mei, YANG Bin, REN Jun, HUANG Lu-sheng (Base Area of National Key Laboratory of Animal Biotechnology, Jiangxi Agricultural University, Nanchang 330045) Abstract: 【Objective 】 The aim of this study was to detect quantitative trait loci (QTL) for the lengths and the diameters of porcine limb bones, the lengths and the diameters of femoral and humerus medulla antra and the thicknesses of the femoral and the humerus walls. 【Method 】 Lengths of 29 limb bones, diameters of 6 limb bones, diameters and lengths of femoral and humerus medulla antra and the thicknesses of femoral and humerus walls were measured in a white Duroc × Erhualian intercross. A whole-genome scan was performed by an interval mapping based least-squares linear regression with 183 informative microsatellite markers. 【Result 】 Fourteen 1% genome-wide significant QTL, Fourteen 5% genome-wide significant QTL and 47 suggestive