基于sf9昆虫细胞的蓝光诱导表达系统的构建_论文.doc

本科毕业论文(设计) 题 目 基于sf9昆虫细胞的蓝光诱导表达系统的构建 Construction of Blu-ray Induced Expression System in sf9 insect Cells 姓 名 学 号 学 院 生命科学技术学院 专业班级 生科1101 指导教师 职 称 研究员 中国·武汉 二〇一五 年 六 月  华中农业大学士学位论文 Construction of Blu-ray Induced Expression System in sf9 insect Cells 学生姓名: 蔡泽蕲 学生学号: 2011304200107 学生专业: 生物学基地班 指导教师: 徐富强 教 授 指导小组: 吴 阳 博后 华中农业大学生命科学技术学院 二一年六’s application in protein expression and the preparation and purification has many advantages over others. Sf9 insect cells is a platform baculovirus expression system . Baculovirus expression system is more and more widely applied in eukaryotic expression system in recent years, which can carry a variety of foreign genes need to express in insect cells, including fungi, plants, bacteria, virus genes. Baculovirus is double-stranded DNA virus,the natural reservoir of which is insect cell.Since has a high degree of species specificity, it not infected vertebrates, positively harmless to human and animal. The present study is more alfalfa crazing armyworm nuclear polygonal body virus (AcMNPV). OpIE1 and OpIE2, two promoters of the vector pMIB/v5 - His which we used to construct the optogenetic gene expression system are early promoter of a baculovirus, and former promoter is more stronger. The baculovirus is Orgyia pseudotsugata multicapsid nuckear polyhedrosis virus (OpMNPV),the natural host of which is the Douglas fir tussock moth.In spite of this, the two promoters will still function in the sf9 cells, sf21, LD652Y and other insects to the drive gene expression. Baculovirus early promoter can drive gene expression of cells in the absence of viral factors . So to some extent, the pMIB vector we used also has the advantages that baculovirus expression vector has. Such as: 1) Folding correctly of recombinant protein , and the formation of disulfide bond; 2) Able to be modified after translation processing ,including: glycosylation, phosphorylation, amidation and si