stat4入核子机制的初步研究.pdf

下载文档 降价啦

1
0
约8.22千字
约 59页
2016-03-04 发布于贵州
举报
版权申诉
保障服务

stat4入核子机制的初步研究.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

stat4入核子机制的初步研究

Y2195093fM andactivatorsof2．transducerslocation1． 42．3．3．1．结论：下，能够引导STAT4入核。关键词：dsNLS，STAT4，入核机制 III waswellwhichwerewaswaswithNuclearABSTRACTWhethermechanismmechanismmolecularDNADNADNAbebioinf01T11atics．aasasaTransducersTonotnotonnuclearnuclearnuclearnuclear．nuclearnuclearnuclearnuclearatCanCanCanandafterSTATsSTATSTAT3STAT4STAT4STAT4STATSTATsSTAT4ofownofofofofofotherordertotoactivatorsaccumulatedSTAT42．SeveralforfactorstoconductcertainconstructedconstructedfoundfindthethethethedomaindomaindomainIVthatthethatthethetheconstructtransducestimulatedtransduction．IttranslocationsomestimulatedIL．12．isisinisinitsintoininduceisinininsertedintolocation11 waswaswaswerewaswerewerewerewaswerewerewerewerewaswildwaswaswithNLSVDNADNADNADNAusedasaaTThesevectorrestrictionCaskirestrictionCaskiretardednamedSTAT4aminoacidsSV40andandaminoacidsSTAT4STATs．andandandSTAT4STAT4-Del26．Classicofofobserved6of2．Thefluorescencefounddeleted395—4cellschecked3．Abovecells．Aftercellscells395-4domainconstructedcellsdetectedcellscellsfoundthesetransfectedtransfectedthetransfectedtransfectiontransfectedthetransfectedthatthetransfectedconfirmedthisthatstimulatedstimulatedIL一1ItIL-1IL一1confirmedinsertedintoinsertedintoinimmunoblot．intoininisininintointoin111．1immunoblot． waswildwaswaswaswithNLSVIaEGFP—STAT4一DelThenuclearnuclearrestorednuclearnuclearatSTAT4atataboveSTAT4一Del．acidSTAT4afterofof6of2．foundclassic395-4thattranslocatedstillstimulationstimulationIL一1locatedinintoinininto1 VlI89 NF．r．BNLSNPCsActiveWBWesternmaintenancemononuclearVIIIBPCRPHAFluorescentProteinFactorKinasebloodblotPBMCGASGFPTGreenTheGammaCTLnuclearnuclearnuclearChainReactionSTATactivatedactivatorsfactor．1factorandchromosomal3celltransducerssiteIFNIRF．1ISGF．3IL一12IL．12RInterferonIFN．inducibleInterferon．stimulatedInterleukinInterleukinlocalization11212 KBfactorlocation AmodeIWhile2canforinstate．NF—KBinactivatedintoanthenucleus． modelmaintenanceTheanulearnuc