efficacy of select disinfectants at inactivating ranavirus.pdfVIP

Vol. 84: 89–94, 2009 DISEASES OF AQUATIC ORGANISMS Published April 6 doi: 10.3354/dao02036 Dis Aquat Org Efficacy of select disinfectants at inactivating Ranavirus 1 2 3 2, 3,* Laura K. Bryan , Charles A. Baldwin , Matthew J. Gray , Debra L. Miller 1University of Georgia, College of Veterinary Medicine, Athens, Georgia 30602, USA 2Veterinary Diagnostic and Investigational Laboratory, University of Georgia, 43 Brighton Road, Tifton, Georgia 31793-1389, USA 3Center for Wildlife Health, Department of Forestry, Wildlife and Fisheries, University of Tennessee, 274 Ellington Plant Sciences Building, Knoxville, Tennessee 37996-4563, USA ABSTRACT: Ranavirus can cause disease in reptiles and amphibians. Because survival time outside of a host remains uncertain, equipment must be disinfected to prevent transmission of ranaviruses. However, disinfectant efficacy against amphibian ranaviruses has not been investigated for chlorhexidine (Nolvasan®), sodium hypochlorite (bleach), or potassium compounds. Our goal was to determine the efficacy of Nolvasan® (0.25, 0.75 and 2.0%), bleach (0.2, 1.0, 3.0 and 5.0%), and Virkon S® (1.0%) at inactivating Ranavirus at 1 and 5 min contact durations. Potassium perman- ganate (KMnO4) (2.0 and 5.0 ppm) was also tested with a 60 min contact time. Nolvasan® at 0.75 and 2.0% and bleach at 3.0 and 5.0% concentration were eff