Procedures for large-scale metabolic profiling of serum and.pdfVIP

PROTOCOL Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry 1–3 2,4 2 2 2 2 Warwick B Dunn , David Broadhurst , Paul Begley , Eva Zelena , Sue Francis-McIntyre , Nadine Anderson , 2 5 2 6 6 7 Marie Brown , Joshau D Knowles , Antony Halsall , John N Haselden , Andrew W Nicholls , Ian D Wilson , 2 1, 2 Douglas B Kell , Royston Goodacre The Human Serum Metabolome (HUSERMET) Consortium 1Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK. 2School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK. 3Centre for Advanced Discoveries and Experimental Therapeutics, Manchester Biomedical 4 Research Centre and School of Biomedicine, Manchester, UK. Department of Medicine, Katz Group Centre for Pharmacy Health, University of Alberta, Edmonton, 5 6 Alberta, Canada. School of Computer Science, The University of Manchester, Manchester, UK. Department of Investigative Preclinical Toxicology, GlaxoSmithKline, Hertfordshire, UK. 7Department of Clinical Pharmacology, Drug Metabolism and Pharmacokinetics, AstraZeneca, Cheshire, UK. Correspondence should be addressed to W.B.D. (warwick.dunn@manchester.ac.uk). Pu