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letters to nature
0
non-viable. Ref2 is involved in snoRNA and mRNA 3 -end for-
The yeast Rat1 exonuclease mation13,14. Synthetic slow growth phenotypes were seen with
mutants of several transcription factors implicated in elongation
0
promotes transcription termination or 3 processing. These include Spt4, the Elongator complex (ELP1-
6), members of the Paf complex, Bur2, Htz1 and its assembly
by RNA polymerase II complex SWR-C, and several ubiquitin/proteasome-related pro-
teins. Finally, synthetic lethality was observed with a deletion of
1 2 1 3 RAI1, which encodes a component of an RNA degradation complex
Minkyu Kim , Nevan J. Krogan , Lidia Vasiljeva , Oliver J. Rando ,
Eduard Nedea2, Jack F. Greenblatt2 Stephen Buratowski 1 (see below).
Tandem affinity purification (TAP) of Rtt103 was used to identify
1Department of Biological Chemistry and Molecular Pharmacology,
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