塞来昔布联合氟嘧啶对人胰腺癌细胞株sw1990生长抑制作用及其机制研究.pdf

英文摘要 ⅢⅢ哪ⅢY 舢2洲3删3㈣8Ⅲm1 川3Ⅲ6 Effectof withFluorouracil The CelecoxibCombined on Inhibitory SWl990PancreaticCancerCellsLine Abstract the effectandthemechanismof inhibitory celecoxib，a Objective：Toinvestigate selectiveCOX一2 fluorouracilonthe of cancer inhibitor，combined谢mgrowthpancreatic celllineSWl990． Methods：The wasdividedintothecontrol fluorouracil experiment group，the different celecoxibandthetwo combined the group，the group drugs group，and concentrationsofthe were on cancercelllinesSW1990．MTT drug actingpancreatic to the inhibitionrateofcellsineach to Wasuseddetect group，andexplore assay growth Wasusedto thebestcell inhibitionof concentration．Flow produce growth drug cytometry detectthe ofdifferenteffectsoncell ofSurvivincells impact drug cycle．Theexpressions ineach WasmeasuredRT—PCR． group by inhibitedthe ofSWl thesurvival Results：Fluorouracil，celecoxibgrowth 990，and cells wimthe oftheconcentrationofthe rateofthe decreased increasing drug．The combinationhadmore inhibitionofcell two—drug group pronounced growth．The celecoxib fluorouracilandthe combinationincreased