a proteomics approach to the identification of biomarkers for psoriasis utilising keratome biopsy：蛋白质组学方法鉴定生物标志物用于银屑病利用角膜活检.pdfVIP

J O U R N A L O F P R O T E O M I C S 9 4 ( 2 0 1 3 ) 1 7 6 – 1 8 5 A v a i l a b l e o n l i n e a t w w w . s c i e n c e d i r e c t . c o m ScienceDirect w w w . e l s e v i e r . c o m / l o c a t e / j p r o t A proteomics approach to the identification of biomarkers for psoriasis utilising keratome biopsy a,⁎ b a b James C. Williamson , Peter Scheipers , Veit Schwämmle , John R. Zibert , Hans Christian Beckc, Ole N. Jensena aDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense DK-5230, Denmark bLEO Pharma A/S, Industrieparken 55, Ballerup 2750, Denmark cCenter for Clinical Proteomics, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Sdr. Boulevard 29, Odense 5000, Denmark A R T I C L E I N F O A B S T R A C T Article history: The discovery of plasma biomarkers for psoriasis vulgaris may aid clinicians in disease grading Received 9 May 2013 and monitoring of treatment response. We have therefore developed a proteomics/mass Accepted 11 September 2013 spectrometry based workflow which enables biomarker discovery from psoriasis patient Available online 20 September 2013 samples. We have utilised keratome skin biopsy, which results in reduced cellular complexity compared to punch biopsy. Furthermore, we applied short ter