a protocol for rna methylation differential analysis with merip-seq data and exomepeak rbioconductor package：一种中东研究与情报计划数据和exomepeak rbioconductor包序列rna甲基化差异分析协议.pdfVIP

Methods 69 (2014) 274–281 Contents lists available at ScienceDirect Methods journal homepage: www.else /locate/ymeth A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package Jia Meng a,⇑, Zhiliang Lu a, Hui Liu b, Lin Zhang b, Shaowu Zhang c, Yidong Chen d,e, Manjeet K. Rao e,f, Yufei Huang g,d,⇑ a Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou 215123, China b School of Information and Electrical Engineering, China University of Mining and Technology, Xuzhou 221116, China c School of Automation, Northwestern Polytechnical University, Xi’an 710072, China d Department of Cellular Structural Biology, University of Texas Health Science Center at San Antonio, TX 78229, USA e Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio, TX 78229, USA f Department of Epidemiology and Biostatistics, University of Texas Health Science Center at San Antonio, TX 78229, USA g Department of Electrical and Computer Engineering, University of Texas at San Antonio, TX 78249, USA a r t i c l e i n f o a b s t r a c t Article history: Despite the prevalent studies of DNA/Chromatin related epigenetics, such as, histone modifications and Received 28 March 2014 DNA methylation, RNA epigenetics has not drawn deserved attention until a new affinity-based sequenc- Revised 14 June 2014 ing approach MeRIP-Seq was developed and applied to survey the global mRNA N6-methyladenosine Accepted 19 June 2014