a novel liquidliquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus：一种新的液-液萃取过程由表面活性剂和乙腈从榴莲的多聚半乳糖醛酸酶酶的纯化.pdfVIP

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a novel liquidliquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus：一种新的液-液萃取过程由表面活性剂和乙腈从榴莲的多聚半乳糖醛酸酶酶的纯化.pdf

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a novel liquidliquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus：一种新的液-液萃取过程由表面活性剂和乙腈从榴莲的多聚半乳糖醛酸酶酶的纯化

Journal of Chromatography B, 993-994 (2015) 1–8 Contents lists available at ScienceDirect Journal of Chromatography B j o u rn al h omepage: w w w. elsev /locate/chro mb A novel liquid/liquid extraction process composed of surfactant and acetonitrile for puriﬁcation of polygalacturonase enzyme from Durio zibethinus Mehrnoush Amid a,∗ , Yazid Manap a,b , Farhana Azmira a , Muhaini Hussin a , Zaidul Islam Sarker c a Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia b Halal Products Research Institute, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia c Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia a r t i c l e i n f o a b s t r a c t Article history: Polygalacturonase is one of the important enzymes used in various industries such as food, detergent, Received 7 September 2014 pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfac- Accepted 23 April 2015 tant and acetonitrile was employed for the ﬁrst time to purify polygalacturonase from Durio zibethinus. Available online 1 May 2015 The inﬂuences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polyga- Keywords: