a simple and sensitive fluorescence based biosensor for the determination of uric acid using h2o2-sensitive quantum dotsdual enzymes：一个简单的和敏感的基于荧光用h2o2敏感的量子dotsdual酶测定尿酸生物传感器.pdfVIP

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a simple and sensitive fluorescence based biosensor for the determination of uric acid using h2o2-sensitive quantum dotsdual enzymes：一个简单的和敏感的基于荧光用h2o2敏感的量子dotsdual酶测定尿酸生物传感器.pdf

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a simple and sensitive fluorescence based biosensor for the determination of uric acid using h2o2-sensitive quantum dotsdual enzymes：一个简单的和敏感的基于荧光用h2o2敏感的量子dotsdual酶测定尿酸生物传感器

Biosensors and Bioelectronics 67 (2015) 129–133 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios A simple and sensitive ﬂuorescence based biosensor for the determination of uric acid using H O -sensitive quantum dots/dual 2 2 enzymes Nur Ellina Azmi a,b, Noor Izaanin Ramli a, Jaafar Abdullah b,c,n, Mohammad Azmi Abdul Hamid d, Hamidah Sidek a, Samsulida Abd Rahman a,c, Nurhayati Arifﬁn a, Nor Azah Yusof b,c a Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato ’ Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia b Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia c Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia d School of Applied Physics, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia a r t i c l e i n f o a b s t r a c t Article history: A novel optical detection system consisting of combination of uricase/HRP–CdS quantum dots (QDs) for Received 12 May 2014 the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal Received in revised form ﬂuorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish 15 July 2014 peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hy