原创力文档a simple electrochemical method for the detection of atp using target-induced conformational change of dual-hairpin dna structure：一种简单的电化学方法检测双发夹结构的靶诱导构象变化.pdfVIP
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a simple electrochemical method for the detection of atp using target-induced conformational change of dual-hairpin dna structure：一种简单的电化学方法检测双发夹结构的靶诱导构象变化
Sensors and Actuators B 222 (2016) 1090–1095
Contents lists available at ScienceDirect
Sensors and Actuators B: Chemical
j o u r n a l h o m ep a g e: w w w. elsev /locate/snb
A simple electrochemical method for the detection of ATP using
target-induced conformational change of dual-hairpin DNA structure
Jing Jia, Ji Feng, Hong Guo Chen, Hong Qun Luo ∗ , Nian Bing Li ∗
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest
University, Chongqing 400715, PR China
a r t i c l e i n f o a b s t r a c t
Article history: A novel method based on both insertion approach for the repeatability improvement and the dual-hairpin
Received 12 May 2015 structure as a signal amplifier for the sensitivity improvement was investigated for adenosine triphos-
Received in revised form 29 July 2015 phate (ATP) detection. For the preparation of the aptasensor, the moiety and methylene blue (MB) labeled
Accepted 10 August 2015
aptamer probe were inserted into a loosely packed cyclic-dithiothreitol (DTT) monolayer, and the MB
Available online 14 August 2015
labeled adjunct probe could hybridize with the aptamer probe to form the dual-hairpin structure on the
electrode. In the absence of the target, the stable dual-hairpin conformation enabled the MB to stay close
Keywords:
to the electrode surface, generating a strong current si
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