a simple bactericidal antibody test for sero-diagnosis of typhoid fever：一个简单的杀菌伤寒血清学诊断抗体试验.pdfVIP

Journal of Immunological Methods, 42 (1981) 121-125 121 0 Elsevier/North-Holland Biomedical Press Short communication A SIMPLE BACTERICIDAL ANTIBODY TEST FOR SERO-DIAGNOSIS OF TYPHOID FEVER ASHOK K. GUPTA and KOLA M. RAO’ Department of Microbiology, Defence Research and Development Establishment, Gwalior-474002, India ’ (Received 15.October 1980. accented 12 November 1980) Serum samples were collected from 24 confirmed cases of typhoid fever, 15 clinically suspected cases and 23 normal healthy controls. The convalescent sera were obtained in 13 of the 24 confirmed typhoid cases. In all, 13 paired sera, 11 acute phase only, 15 clinically suspected and 23 normal serum samples were tested for eliciting bactericidal antibodies to Salmonella typhi. In addition, the Widal teat was also performed for com- parison. All the 24 acute phase sera aa well as 13 convalescent sera were found to be positive by bactericidal antibody test (titre 180 or above). Of 15 clinically suspected cases, 5 were positive whereas one of the 23 normal controls sera gave a false positive reaction. In contrast, the Widsl test could detect only one of the 24 cases in the acute stage, but all 13 cases showed antibodies at a diagnostic titre level during the convalescent stage. None of the 15 clinically suspected cases or 23 normal controls were positive by the Widal test. The feasibility of using a bactericidal antibody test in sero-diagnosis of typhoid fever is discussed. INTRODUCTION Enteric fever in man occurs throughout the world. In India, most of the infections are due to Salmonella typhi (Agsrwal, 1963; Nath et