let-7c对癌细胞增殖的影响及其机制.pdfVIP

目 录 中文 摘 要 英 文 摘 要 一 月叮舀 ··.一 ’’‘二 ‘” ·“ 二‘ “二 ’‘‘·… ’二 “二’. ·… ‘·… ’·’二 ”. “. ’二 “”.… ”” ’‘.‘“”” ”. ’二 “. 第 一 部 分 let一7c 对 HCC LM3 细 胞 增 殖 的影 响 材 料 与 方 法 ··············································································… …6 结 果 ·······················································································… …8 小 结 ····················，··································································… … 13 第 二 部 分 应 用 生物 信 息 学 手 段 预 测 let 一7c 的靶 基 因 方 法 ·······················································································… … 14 结 果 ··············································，··，·，···································… … 14 小 结 ···························································，···························… … 16 第 三 部 分 let一7C 调 控 eyelin D I、Cye lin DZ 以及 CDK6 的表达 材 料 与方 法 ··············································································… … 18 结 果 ·······················································································… …26 小 结 ····························，····················，················，····，···············… … 32 全 文 讨 论 ···，·············································································… …33 参 考 文献 ·······················，······················，··································……36 综 述 ·················，·····································································… …38 附件 ·······················································································… …44 致 谢 ··············································，········································… …45 论 文 声 明 ······································，·，·····························，··········… …46 硕 士 学位 论 文 中文 摘 要 背 景和 目的 M irc 0R N A (mi R N A ) 是 一种 约 22 个 核普 酸 长 度 的小 分子 单 链 R N A 。在 肿 瘤 中 ，与周 围正 常 组 织 相 比 ，一 些 m iR N A 发 生 异 常 表 达 。研 究发 现 这 些 异 常 表 达 的 m iR N A 能影 响肿 瘤 的增 殖 、