人参皂甙rg3乳腺癌mcf-7细胞凋亡的影响.pdfVIP

人参皂甙l蟛对乳腺癌MCF．7细胞凋亡的影响 中文摘要 目的探讨人参皂甙I沁3对乳腺癌McF．7细胞凋亡的影响 方法实验分为对照组，人参皂甙I迫3实验组。采用MTT法观察人参皂甙R93对乳腺癌 细胞FaS的mRNA表达 时，MCF一7细胞的生长抑制率随R93浓度的增加而增大，与对照组比较，细胞增殖受到明 表达。 CaSpase-8，CaSpaSe．3酶活性，增加乳腺癌MCF．7细胞凋亡。 关键词人参皂甙R93，凋亡，乳腺肿瘤，MCF．7细胞，CaSpase．8 山两医科大学硕士学位论文 Theinnuenceof toMCF一7cell ginsendoside—R窀r3 apoptosis inbreastcancer Abstract T0researchthe砌uenCeof toMCF一7ceU iIlbreast objective giIlsendoside-R93apoptosis CanCef MethodA1lthe diVidedirltotwo controland subject、Ⅳere groups，includinggroupexperiIIlent of irlhjbitionof onthe ofMCF．7cells、ⅣasdetectedMTT groupR93．The R93 growch by assay， wascalculatedtoobt血thee丘-ective IC50 concemration；CaSpase．8，CaSpase．3enzymesaCtivity of ofFaslll】ⅢAwaSdetected MCF．7cells、Ⅳasdetectedbycolor曲etric：Theexpression by IoPCRtecIlIlo molecularlevel． logy疗om ResunsThe of theconcentrationof IC50R93、惴(200士0．712)“g埘．mlen R93、惴a盯a119ed rate cellswas the 丘om37．5to ofMCF-7 iIlllibitory lligherfollowing 600．0“g／IId，thegro嘶h iIlcreaseof“sconCentration．Thecell mhibited withcontrol growchwas伊eatly compared group iIl (P0．05)；ColorhnetricdisplayCaSpause一8，CaLspase一3enzyIneactiVity、Vashigherexperimem thanthatiIlcontrol the“刚AFas 黟oup group(PO．05)；Im