1株具解磷特性大豆根瘤菌的分离筛选和回接鉴定.pdfVIP

微生物学杂志 2010 3 30 2 JOURNA OF M ICROBIO OGY M ar. 2010 Vo.l 30 No. 2 51 1 韩 梅, 于 芳, 肖亦农, 黄玉茜, 韩晓日* (, 110866) 从沈阳农业大学的科研基地分离得到 20株根瘤菌, 一步利用乙炔还原法和溶磷圈法得到 1株兼 - 1 - 1 具有结瘤固氮和解磷作用的根瘤菌# 6 菌株# 6的固氮酶活性为 4. 94 molC H g m in , 与参照菌株 2 4 USDA 110的固氮酶活性相当;用溶磷圈法定性试验# 6解磷性, 其可以溶解有机磷, 对照菌株 USDA 110无解磷 现象; 一步测定# 6的解磷量, 其解磷量分别在第 7天为 35. 50 g/m , 第 14天为 21. 05 g/m ; 对根瘤菌# 6 生理生化和生长特性 行试验, 以利于实际应用 根瘤菌; 固氮; 解磷 ; 分离; 筛选 Q93- 331 A 1005- 7021( 2010) 02- 0051- 06 Isolation and Screening of a Phosphate DissolvingRh izob iium j ap on icum Strain and Its Characterization by Back Inoculation HAN M ei, YU Fang, X IAO Y inong, HUANG Yuqian, HAN X iaori (Sh eny ang A g ric. Uni. , Sh enyang 110866) A bstract 20R h izob ium strainswere isolated from soybeans in the scientific research base of Shenyang Agricu ltural University. ARA and phosphate solubilization circlem ethod ( PSCM ) w ere used further to obtainR h izobium strain# 6 w ith nodu leform ingandnitrogenfixing and phosphate d issolving effects. Its n itrogenase activity was at 4. 94 m ol C H /m in/ g being equ ivalent to that of the reference strain USDA 110. Its phosphorus d issolving ab ility w as tested 2 4 w ith PSCM andw as found it could dissolve organ ic phosphorous; w hile the reference strain USDA 110 could not. Fur ther determ ination of# 6 s phosphate dissolving quantity respectively w ere 25. 2g /m at 7th d and 21. 05 g /m at 14 th d. Finally its physiological, b iochem ica,l and grow th characteristicsw ere tested to the end that helpful for practi cal application. K eywords R h izobium; nitrogenfixing; phosphate solub ilization; isol