右旋美托咪啶对鼠镇痛作用及其机制的研究.pdf

右旋美托咪啶对大鼠镇痛作用及其机制的研究 摘 要 目的： 探讨右旋美托咪啶(Dexmedetomidine，Dex)对切口痛大鼠 的镇痛作用以及对脊髓nNOS及SP表达的影响。 方法：雄性Wistar大鼠48只随机分为4组，每组12只。A组不做手术； B、C、D组于左后爪作一纵形切口制备切口痛模型；C、D两组分别于术前 10min腹腔注射Dex50g/kg、30g/kg。观察术后2h、6h、24h、48h的累 积疼痛评分(CPS)和机械缩爪阈值(MWT)。每组随机选取6只大鼠于术后2h 处死取材，采用免疫组化及RT-PCR方法检测脊髓nNOS、SP表达，并进行 计算机图像分析。所有结果经SPSS11.0统计分析，P0.05被认为有统计 学意义。 结果：C、D组术后CPS较B组明显降低，MWT明显升高，两组与B 组比较在术后各时间点均有统计学差异（P0.05）。C组镇痛强度高于D 组，两组在术后2h、6h比较有统计学差异（P0.05）。免疫组化和RT-PCR 检测结果显示， B组中nNOS及SP的蛋白和mRNA表达呈强阳性，C、D 组表达呈弱阳性，两组与B组比较有统计学差异（P0.05）。C组 nNOS 及SP的蛋白和mRNA表达弱于D组，两组比较有统计学差异 （P0.05）。 结论： 右旋美托咪啶对切口痛大鼠有明显镇痛作用，且 50g/kg 镇痛强度大于30g/kg，其镇痛作用可能与抑制脊髓nNOS、SP表达有关。 关键词 右旋美托咪啶，切口痛，2肾上腺素能受体激剂， nNOS，SP THE ANALGESIC EFFECTS AND MECHANISM OF DEXMEDETOMIDINE ON RATS ABSTRACT Object: To investigate the analgesic effects of dexmedetomidine and neuronal Nitricoxide Synthase and substance P expression in the spinal cord on rat incisional pain model. Method: Forty eight male wistar rats were randomly divided into 4 groups and 12 rats in each group. The Group A didn’t operated, while the group B、C 、D received a longitudinal incision in the left hindpaw to make pain model. The group C and D were peritoneal injected dexmedetomidine 50g/kg and 30g/kg at 10 minutes before operation. Cumulative pain score (CPS) and mechanical withdrawal threshold (MWT) were measured at 2h,6h,24h and 48h. The six rats in each group were sacrificed at 2h to detect nNOS and SP expression in the the spinal cord by immunohistochemistry and RT- PCR. Moreover, image analysis was used to quantify the data. All the results were statistically analyzed by SPSS 11.0 software. A p value less than 0.05 was considered statistically significant. Result ： The rats got lower CPS and higher MWT