sirna沉默62基因对人肝癌细胞hep3b的生物学影响.pdf

摘 要 互关系。 2000转染。 首先从3条siRNA序列中筛选出l条沉默p62最佳的序列。然后将细胞分为空 和Western 白质的表达量，并用MTT检测细胞增殖能力的变化。 。 siRNA 200_中组 结果：沉默p62最佳的siRNA序列为Si．h．GTF2HIp62 的mRNA表达较其他3组明显上调。Western blot检测结果显示各组细胞中p62、 测显示siRNA沉默p62后，细胞增殖力增强。 并且可能通过DNA损伤检控点来调控细胞增殖。 关键词肝细胞癌RNA干扰基因p62转染细胞周期 Il Abstract ABSTRACT the effectson cell of objective：To biological hepatomaHep3B investigate useof siRNA． p62by p62 Methods：Theweretransfectedinto cell with siRNAs hepatomaHep3B bestsiRNA whichsilent was Lipofectamine2000．First，the sequence p62 fromthree as selected siRNA targetingp62(named sequencesspecifically and Si—h-GTF2H1—001，Si—h-GTF2H1—002 were control classifiedfour group：2,siRNAgroup： exprement groups：1,blank of expression were RT-PCRandWesternblot．Thecell detected p62，XPD，p53，cyclinDlby wereexaminedMTT． cycle by Results：ThebestsiRNA whichsilent wasSi··h·· sequence p62 withblankcontrol and GTF2H1—002．Contrast group，N