响应面法优化人参叶总皂苷提取工艺的研究.pdfVIP

第37卷摇 第2期 北 京 林 业 大 学 学 报 Vol.37,No.2 2015年2月 JOURNAL OF BEIJING FORESTRY UNIVERSITY Feb. ,2015 DOI:10.13332/ ki.jbfu.2015.02.003 响应面法优化人参叶总皂苷提取工艺的研究 程摇 晶摇 雷建都摇 刘摇 静摇 王璐莹摇 邓立红摇 何摇 静 (北京林业大学材料科学与技术学院,林木生物质化学北京市重点实验室) 摘要:为优化人参叶总皂苷提取工艺,采用单因素试验,考察温度、时间、液料比对人参叶中总皂苷得率的影响。 再 采用3 因素3水平的响应面分析法确定人参叶总皂苷提取的优化工艺,同时建立人参叶总皂苷提取的二次项数学 模型,并验证其可靠性。 结果表明:热水浸提法提取人参叶总皂苷的最佳条件为温度 100 益、时间4郾2 h、液料比 16。 在最佳条件下,人参叶总皂苷得率为人参叶质量的14郾23%。 关键词:人参叶;皂苷;提取;响应面分析法 鄄鄄 鄄鄄 鄄鄄 中图分类号:R284郾2摇 摇 文献标志码:A摇 摇 文章编号:1000 1522(2015)02 0142 06 CHENGJing; LEI Jian鄄du; LIU Jing;WANG Lu鄄ying; DENG Li鄄hong; HE Jing. Optimization of extraction technology of ginsenosides from ginseng leaves by response surface method. Journal of 鄄鄄 Beijing Forestry University (2015)37 (2)142 147 [Ch, 14 ref.]College of Materials Science and Technology,Beijing Key Laboratory of Lignocellulosic Chemistry,Beijing Forestry University,Beijing, 100083,P. R. China. In order to optimize the extraction process of total ginsenosides from ginseng leaves, firstly we utilized one鄄factor experiment to investigate the influences of the factors (temperature,treatment time, and liquid 鄄material ratio) on the extraction yield of total ginsenosides. Then we determined the optimal extraction process by the response surface methodology with 3 factors and 3 levels,and established the quadratic mathematical model for the ginsenosides extraction, and finally verified its reliability. The optimal extractionconditionsforthehotwaterextractionoftotalginsenosideswereasfollows:temperature 100 益, treatment time 4郾2 h, liquid鄄material ratio 16. Un