参考文献[references]-应用与环境生物学报.docVIP

醋酸杆菌生物催化丙烯醛合成丙烯酸( 王旭昌1 宗红1 陆信曜1 诸葛斌1 方慧英1** 宋健2 诸葛健 1江南大学工业生物技术教育部重点实验室，生物工程学院，工业微生物研究中心??无锡??214122 摘 要 本文开展了生物催化丙烯醛生产丙烯酸的研究。通过菌株筛选和获得株目的微生物，经鉴定比较发现Acetobacter sp.在Acetobacter sp.细胞浓度15 g/L，初始底物浓度为10 g/L，pH 6.8，30 的条件下反应1 h，转化率可达 88.0%，产物浓度达到11.32 g/L 丙烯，2.5 h的反应，20 g/L的丙烯醛被催化成22.96 g/L丙烯酸，摩尔转化率为89.3%，固定化细胞仍达到%。利用Acetobactersp.细胞催化生产丙烯酸，为丙烯酸的制备提供了一种新的可能。图8参15 关键词 Acetobacter sp.，全细胞催化，丙烯醛，丙烯酸，固定化细胞 CLCQ819 Biosynthesis of Acrylic Acid from Acrolein by Acetobacter sp.* Xuchang Wang1, Hong Zong1, Xinyao Lu1, Bin ZhuGe1, Huiying Fang1**, Jian Song2 Jian Zhuge1 1Key?Laboratory?of?Industrial?Biotechnology,?Ministry?of?Education,?Jiangnan?University,?Laboratory?of?Industrial?Microorganisms,?School?of?Biotechnology,?Jiangnan?University,?Wuxi?214122,?China 2School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China Abstract To obtain a screen strain with capacity of transforming acrolein to acrylic acid, two strains were isolated and identified by morphological analysis, biochemical properties and 16S rDNA gene sequences. Acetobacter sp. exhibited higher catalytic performance. At optimum conditions (cell concentration 15 g/L , acrolein concentration 10 g/L, pH 6.8, 30 ℃) , 11.32 g/L acrylic acid were produced and molar transcoversion was 88.0%. Immobilized cells method was applied to enhance the acrolein resistance of the strain. Sodium algium of 2% and CaCl2 of 0.4% were used to entrapped Acetobater cells. In this way, 20 g/L acrolein were catalysted to 22.96 g/L acrylic acid in 2.5 h and molar conversion reached 89.3%. After 6 times reused, 85.4% productivity were observed. This study provide a promising alternative of acrylic acid production. Keywords Acetobacter sp.; whole cell catalyst; acrolein; acrylic acid; immobilized cells 丙烯酸是一种重要的有机化工产品，主要用于合成丙烯酸酯类及其聚合物[1,2]。目前，丙烯酸的生产方法主要为化学合成法，其中以丙烯氧化法为主[3]，但化学合成法具有能耗大、成本高等缺点。而生物法具有反应条件温和、环境友好、副产物少、操作简单研究Gartner[4]发现Clostridium propionicum在厌氧发酵过程中可将乳酸直接还原为丙烯酸，产量仅为0.79 g/L；Akedo[5]Sansever