小鼠胚胎干细胞分化成中脑神经元.pdfVIP

© 2000 Nature America Inc. • TECHNICAL REPORTS generation of embryoid bodies (EBs; stage 2), the use of a defined Efficient generation of medium to select for CNS stem cells (stage 3), the proliferation of CNS stem cells in the presence of mitogen, basic fibroblast growth factor (bFGF; stage 4), and the differentiation of the stem cells by midbrain and hindbrain removal of the mitogen (stage 5; Fig. 1A). The successful conversion of ES cells into midbrain dopamin- ergic (DA) neurons in vitro should be dependent on the induction neurons from mouse of the same genes that are expressed in CNS stem cells and neurons in vivo. The OTX homeobox genes (OTX1 and OTX2) are widely expressed at early stages of neurectoderm differentiation16. OTX2 embryonic stem cells