六神丸通过Wn信号转导通路抗肿瘤的临床与实验研究.pdfVIP

Clinical and Experimental Study for the anti-Lung Cancer of Liu-shen Pill By Wnt signaling pathway Speciality: Oncology of TCM Author: Hou qianqian Tutor: Professor Li Xiurong Abstract Objective: To observe the supression of liu-shen Pill on non-small-cell lung cancer and lung cancer cells A549, and further to discuss the mechanism of liu-shen Pill on anti-tumor. Methods: In the clinical researchs, 40 patients with non-small-cell lung cancer patients were randomly divided into treatment group (GP regimen +liu-shen Pill) including 20 patients and the control group (GP regimen) including 19 patients. Changes of the tumor size, clinical symptoms, body weight, KPS score, and side effects between the two groups after two periods of treatment were observed. In the experimental studies, to explore the possible molecular mechanism of inhibiting the proliferation of lung cancer cells by neucleoprotein β-catenin, the key factor of Wnt signaling pathway applying MTT, immunohistochemistry, and RT-PCR. Results: The clinical study confirmed that cancer patients of combination chemotherapy and liu-shen Pill can be much better in the clinical symptoms and general conditions, protect bone marrow to reduce the blood toxicity induced by chemotherapy, but in the stability and reduce lesions ,the impairment of liver and kidney function and toxicity of the digestive system compared with chemotherapy alone was no significant difference. In terms of experimental study confirmed: cells were treated with the serum containing liu-shen, the serum group has slower growth compared with normal mouse serum OD value, The positive rate of β-catenin,s expression of the