慢性胃炎肝郁证鼠模型表征与胃黏膜细胞凋亡及Bax、Bcl-2表达的相关研究.pdfVIP

Research on Exterior Signs of Liver-qi Stagnation Syndrome of Chronic Gastritis in Rats Model and Related Cell Apoptosis and Expressions of Bcl-2 and Bax with Gastric Mucosal Lesions Speciality:Diagnostics of TCM Author:Ren Jian Tutor:Liu Jiayi Abstract With quick life rhythm of people,weighing heavily on the spirit has been a gradual growth.Disease caused by undesirable mood for a long time is on the increase. Chronic gastritis is a common diseases of digestive system. The cause of chronic gastritis is correlated with Overstrain and emotional maladjustment Purpose:Study of the relationship between chronic gastritis liver-qi stagnation syndrome and pathological change of stomach mucosal biopsy in rat models of syndrome and disease with chronic gastritis. Discussion for apoptosis in stomach mucosal cell and the expression of apoptosis-related genes Bcl-2 and Bax in rat models syndrome and disease. Methods: The method and the design about CG Model combining syndrome with disease, establish a model of chronic gastritis liver-qi stagnation syndrome in rats.The normal group, the CG group, the liver-qi stagnation group as control. In 9 weeks, 11 weeks and 12weeks, follows up the changes of exterior signs in the circadian activity rhythm of rats, to test the serum level of NO by colorimetry and ET by ELISA,take stomach for HE dye, observe the histology and morphology under stomach mucosal. The stomach mucosal specimens from executing the treated rats in different group were randomly collected and fixed in formalin and embedded with paraffin. The samples were examined with HE stain,immunocytochemistry techniqu(Bcl-2,Bax)and TUNEL dyeing.After executing the treated rats,the samples