斑蝥素对HEL胞株JAK2基因表达的影响研究及复方斑蝥胶囊治疗真性红细胞增多症临床疗效观察.pdfVIP

The Effection of Cantharidin on Tumor Cell Proliferation in MPN and Clinical Curative Effects of Compound BanMao Capsule to PV Patients Special: Internal Medicine of Integrated TCM Student: Wang Yuhui Tutor: Prof. Xu Ruirong ABSTRACT Part1 Experimental study Objective: Study the influence of cantharidin of Chinese medicine extract to tumor proliferation on HEL cells, and detector the expression of JAK2 gene, in order to know the influence of cantharidin to clarify anti-tumor effect, and expounds the relevance between its mechanism and the expression of JAK2 gene.Methods: By MTT method to affirm the inhibition of proliferation of cantharidin to the HEL cell.The RT-PCR half quantitative method to detect different concentrations of cantharidin HEL cell lines JAK2 gene expression of the influence of the situation.Result: Cantharidin inhibits HEL cell proliferation, significant time and dose effect; JAK2 gene expression of the quantity is all has significant difference between blank group and dose of cantharidin group, blank group and between high doses of cantharidin between high doses of cantharidin and between groups （P0.01 ）;Both dose of cantharidin group and high doses of cantharidin group can reduce the relative expression of JAK2 gene(P 0.01); The relative expression of JAK2 gene of high dose group is less than the dose group (P 0.01).Conclution: The thing that cantharidin can inhibit proliferation of HEL cell lines relevanted to it can reduce the JAK2 gene expression. Part2 Clinical research Objective: Research compound BanMao capsule with western medicine on the clinical Effectiveness in patients with PV.Methods: 40 patients with PV were sorted to two groups of e