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中药复方桃红四汤对大鼠肝星状细胞凋亡的影响
目 录
中文摘要 ···················································1
英文摘要 ···················································3
引 言 ····················································6
正 文 ····················································9
1 实验材料 ················································9
1.1 实验细胞·················································9
1.2 主要实验试剂 ·············································9
1.3 主要试剂的配制 ··········································11
1.4 主要仪器 ················································12
2 实验方法 ···············································14
2.1 MSCs 分离、培养、传代 ···································14
2.2 HSCs 培养、传代 ·········································14
2.3 细胞共培养方法的建立 ····································17
2.4 实验分组················································17
2.5 MTT 法检测HSCs 增值率 ····································17
2.6 DNA Ladder 测定 ··········································18
2.7 RT-PCR ··················································19
2.8 ELISA 法检测 ·············································21
2.9 数据分析 ················································22
3 实验结果 ················································22
3.1 MSCs 的分离培养、扩增 ····································22
3.2MSCs 诱导后形态学变化 ····································22
3.3 HSCs 的形态观察及a-SMA 的表达 ···························23
3.4 桃红四物汤对HSCs 的增殖抑制 ·····························23
3.5 DNA 凝胶电泳（DNA Ladder ）测定··························24
1
3.6 RT-PCR 检测各组HSCs 的Bax mRNA 和Bcl-2 mRNA 表达 ········24
3.7 RT-PCR 检测共培养组中MSCs 的HGF mRNA 表达 ···············25
3.8 ELISA 法检测两共培养组上清液中HGF 浓度 ··················25
4 讨 论 ················································25
4.1 肝纤维化与HSCs、MSCs、HGF ······························25
4.2 MSCs 的分离、培养及鉴定 ··································27
4.3 共培养体系的应用 ········································27
4.4 桃红四物汤抗肝纤维化作用的机理探讨 ······
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