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侧脑室注射抗生抑素血清在炎症痛及针刺镇痛中的作用机制
Intra-cerebroventricular injection of anti-somatostatin-serum
involved in the Inflammatory pain and Electro-Acupuncture
Analgesia in Rats
Speciality:Acupuncture and Moxibustion
Author:Cui Xiaolu
Tutor:Wu Fudong
ABSTRACT
Objective:To investigate the influence of endogenous somatostatin(SS) on the
FAC-indced inflammatory pain and acupuncture analgesia,to investigate their possible
central mechanism.
Methods:AA rats were used as the subjects of the study,by using methods of
behavioral pain threshold test,radioimmunity, ABC-ELISA, HPLC-ECD, immunohisto-
chemistry,and Western blot,we observed AA rats’ pain threshold ,the level of IL-6 and
IL-10 in blood serum,the express of SS,the content of 5-HT and β-EP in hypothalamus and
spinal cord,and the express of Gi in hypothalamus after icro-injection of ASSS into lateral
cerebral ventricles. Observe the changes of pain threshold after injection anti serum of
5-HT and β-endorphin on anti serum of SS or electro-acupuncture.
Results :Using icro-injection of ASSS into lateral cerebral ventricles ,rats’pain
threshold can be reduced,the level of IL-10,the level of 5-HT andβ-EP in hypothalamus
and spinal cord ,the express of SS in hypothalamus also can be reduced,while the level of
IL-6 and the express of Gi-protein in hypothalamus can be increased. If adding injection
the anti-serum of 5-HT and the anti-serum of β-EP ,the pain threshold mostly decreased
than before, and the expression of SS in hypothalamus decreased than before.
Conclusion:The SS may be an important regulative point on inflammatory pain and
acupuncture analgesia. The SS may be related to the 5-HT or β-EP and the IL-6 or IL-10
during the process of e
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