补肾中药生精方肾阳虚男性不育患者精子dna印记基因h19表达的研究.pdfVIP

Born in patients with kidney Yang pure party sperm DNA methylation influence CTmRNA Expression of Research Speciality:Gynecology of TCM Author :guo liang Tutor :Professor Lian Fang Abstract Objective: Watch birth of traditional Chinese medicine pure party nourishing kidney patients with Yang sperm DNA methylation, H19 gene expression state mark, the effect of traditional Chinese medicine to improve less discussed kidney disease patients weak sperm function mechanism of sperm quality. Methods: clinical research 67 patients with less because of weak sperm disease of infertility patients were randomly divided into two groups, with pure party respectively born (even the party to professor from party) deal with 3 months (the treatment group, 34 cases) and traditional Chinese medicine from the Pope pill abital disposal after 3 months (control group, 33 cases were treated and extraction patients sperm positive PCR, the RT-PCR detection H19 gene combination RFLP molecularly imprinted in weak sperm molecularly imprinted in the less disease state; And then testing two groups were symptoms of kidney Yang integral change, before and after treatment routine check of the semen changes, including sperm quantity, vitality, liquefied state, sperm deformity rate, combined with clinical ShouJingLv and pregnancy rates were assessed. Results: （1）Two groups of patients with kidney Yang symptoms were all has been improved, but the treatment group than in the control group curative effect is distinct, before and after syndrome integral is statistically significant difference (P 0.05) （2）The treatment group sperm quantity, vitality, liquefied state, sperm deformity rate was better than control. （3） ShouJingLv clinical treatment group was better than control and pregnancy rates, the