补肾中药对多囊巢综合征卵泡抑素的影响和机理探讨.pdfVIP

The exploration on regulation of FS by kidney-reinforcing drugs in PCOS of Shenxu type Specialty: Gynecology of TCM Author: Zhang Meng Tutor: Prof. Lian Fang Abstract Objective:To explore the pathogenesis of FS in PCOS and regulation of FS by kidney-reinforcing drugs, futher enrich the theory of kidney dominating reproduction.. Methods: Fourty infertile patients with PCOS undergoing IVF-ET were divided into two groups, 20 cases in the study group treated with Erzhi Tiangui Granules and western medicine, and the other 20 cases in the control group were treated with gonadotropin- releasing hormone agonist (GnRH-a) and gonadotropin (Gn) alone. The long term protocol was adopted. The expression of FS in follicular fluid on the day of retrieving oocytes were detected by ELISA, and symptom scores, number of retrieved oocytes were evaluated and evalute the relation between FS and these indexes. Thirty mice were allocated into three groups randomly in experimental research,① normal group ： performed hypodermic injection with sterile water for injection usp of same volume as model group and study group on the same time schedule; ② model group ：performed hypodermic injection with Sodium prasterone sulfate, 9mg/100g weight ③ study group ：the same volume of Sodium prasterone sulfate were given; 10 days later, Erzhi Tiangui Granules were given to study group by gastric perfusion . The other two groups were given same volume salt solution. other 10 days later, all mice were killed. Their vaginal smears, uterus index and overy index, histomorphology of uterus and overy ,serum FS，tissue FSmRNA were observed 。 Results: Kidney yin deficiency were presentated in PCOS pat