补肾活血方治疗骨性关节炎的临床与实验研究.pdfVIP

Clinical and experimental study on treatment of knee osteoarthritis by BuShenHuoXue Formula Speciality：Orthopaedicics of TCM Author ：Qi Xuexi Tutor：Professor Li Gang Abstract Objective ：Under the guidance of the theory of Traditional Chinese Medicine，via clinical study and experimental，this paper is to observe the clinical curative effect of the treatment on postmenopausal knee osteoarthritis(KOA) by the BuShenHuoXue Formula and discuss its possible mechanism of action ． Method: The clinical cases were collected and divided into group of TCM (by the BuShenHuoXue Formula) and group Celeboxib randomly. Every group had 30 cases respective ，which would be treated 8 weeks respectively ．When the treatments were accomplished，we evaluated their clinical curative effect ，the changes of main symptoms and signs and the Chinese physician symptom ．Experimental study, 8-week-old SD rats were randomly divided into two groups, group of five Chinese and western medicine group 3. Provide adequate daily water and food, adaptive feeding for three days. Between animals and humans based on dose conversion, intragastric administration of 8 days, and then blood centrifugation, serum separation, storage backup. Neonatal rabbit chondrocytes were cultured and passaged, and with toluidine blue staining of cartilage cells in the presence of, take 2 chondrocytes experiment, four kinds of matrix metalloproteinase levels were double antibody sandwich enzyme-linked immunosorbent assay (ELISA) determination of absorption Degree of value, and then converted into the actual concentration of sample for analysis. ： ： ． ： Results Clinical study 1 WOMAC OA index the WO